| 李浩1,2,孟加梅3,郭娟1,田波1.一株兼具产油和纤维素降解能力的印度血桐
种仁内生菌的筛选及发酵条件优化[J].中国油脂,2025,50(4):.[LI Hao1,2, MENG Jiamei3,GUO Juan1,TIAN Bo1.Screening and fermentation conditions optimization of endophytes from Macaranga indica seed kernel with both oil production and cellulose degradation capabilities[J].China Oils and Fats,2025,50(4):.] |
| 一株兼具产油和纤维素降解能力的印度血桐
种仁内生菌的筛选及发酵条件优化 |
| Screening and fermentation conditions optimization of endophytes from Macaranga indica seed kernel with both oil production and cellulose degradation capabilities |
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出版日期:2025-04-20
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| DOI:10.19902/j.cnki.zgyz.1003-7969.240086 |
| 中文关键词: 植物内生菌 印度血桐种仁 纤维素降解 微生物油脂 发酵条件优化 |
| 英文关键词:plant endophytes Macarange indica seed kernel cellulose degradation microbial oil fermentation condition optimization |
| 基金项目:云南省基础研究专项重点项目(202101AS070011);云南省中青年学术和技术带头人后备人才项目(202105AC160083) |
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| Author Name | Affiliation | | LI Hao1,2, MENG Jiamei3,GUO Juan1,TIAN Bo1 | 1.Key Laboratory of Tropical Plant Resource and Sustainable Use, Xishuangbanna Tropical Botanical
Garden, Chinese Academy of Sciences, Kunming 650223, China 2.University of Chinese Academy
of Sciences, Beijing 101408, China 3.Pu′er Tea College, West Yunnan University
of Applied Sciences,Pu′er 671006,Yunnan,China |
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| 摘要点击次数: 2131 |
| 全文下载次数: 2058 |
| 中文摘要: |
| 旨在寻找兼具产油和纤维素降解能力的菌株以有效降低发酵成本,进而促进产油微生物的应用。通过苏丹黑B染色法筛选印度血桐种仁中具有产油能力的内生菌,并通过刚果红筛选法初筛、滤纸条崩解实验和产纤维素酶活力测定复筛纤维素降解能力较强的菌株,采用单因素试验对其发酵条件进行优化,并对其产油脂主要脂肪酸组成进行测定。结果表明,印度血桐种仁中共筛选出6株兼具产油能力和纤维素降解的菌株,其中Mi007号菌株的透明圈直径与菌落直径比值(3.16)较大,滤纸崩解情况最佳,产纤维素酶活力(1233 U/mL)最高,其纤维素降解能力最强。优化的发酵条件为酵母粉用量1.0 g/L、pH 6、接种量5.0 mL(40 mL培养基),在此条件下Mi007号菌株有最佳产油量,为409.31 mg/L,所产油脂主要脂肪酸为棕榈酸(C16∶ 0)、硬脂酸(C18∶ 0)和油酸(C18∶ 1)。经测序鉴定,Mi007号菌株为巨大普利斯特氏菌(Priestia megaterium),序列相似度为99.93%。综上,Mi007号菌株兼具产油和纤维素降解能力,且产油量较高,有望成为克服工业发酵成本问题的备选菌株。 |
| 英文摘要: |
| 1.Key Laboratory of Tropical Plant Resource and Sustainable Use, Xishuangbanna Tropical Botanical
Garden, Chinese Academy of Sciences, Kunming 650223, China; 2.University of Chinese Academy
of Sciences, Beijing 101408, China; 3.Pu′er Tea College, West Yunnan University
of Applied Sciences,Pu′er 671006,Yunnan,China |
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