| 王晓杰，杨晶晶.D-氨基葡萄糖修饰玉米肽的
抗运动性疲劳效应[J].中国油脂,2025,50(5):.[WANG Xiaojie, YANG Jingjing.Anti-exercise fatigue effect of D-glucosamine modified zein peptides[J].China Oils and Fats,2025,50(5):.] |
| D-氨基葡萄糖修饰玉米肽的
抗运动性疲劳效应 |
| Anti-exercise fatigue effect of D-glucosamine modified zein peptides |
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| DOI：10.19902/j.cnki.zgyz.1003-7969.240234 |
| 中文关键词: 玉米肽 D-氨基葡萄糖 玉米糖肽 抗疲劳 |
| 英文关键词:zein peptides D-glucosamine zein glycopeptides anti-fatigue |
| 基金项目:黑龙江省省属高等学校基本科研业务费科研创新平台项目（145309330）；黑龙江省植物性食品加工技术优势特色学科“科技攻关”专项项目（YSTSXK202305） |
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| 中文摘要: |
| 旨在为玉米糖肽抗疲劳产品的开发提供理论依据，采用碱性蛋白酶Alcalase水解玉米醇溶蛋白制得玉米肽,再以转谷氨酰胺酶为催化剂，D-氨基葡萄糖为酰基受体，通过酶法糖基化反应修饰玉米肽制备玉米糖肽，以玉米糖肽为受试样品（低、中、高剂量分别为125、250、500 mg/kg)，玉米肽为阴性对照(剂量为500 mg/kg)，对小鼠连续灌胃28 d后，检测小鼠体质量、运动耐力以及疲劳生化指标，考察玉米糖肽对小鼠生长状况的影响及其对小鼠的抗疲劳效应。结果表明：实验剂量的玉米糖肽和玉米肽对小鼠生长状况未产生不良影响；与正常组（灌胃生理盐水）相比，250 mg/kg玉米糖肽组小鼠的转棒时间和负重游泳时间分别显著延长至5.71倍和2.48倍，而玉米肽组小鼠的转棒时间和负重游泳时间均无显著变化；与模型组相比，250 mg/kg玉米糖肽组小鼠血清乳酸脱氢酶活力、乳酸含量、尿素氮含量以及肝脏丙二醛含量分别降低30.29%、13.20%、31.59%、5059%，肝糖原、肌糖原、谷胱甘肽含量以及超氧化物歧化酶和谷胱甘肽过氧化物酶活力分别增加18.38%、54.40%、42.20%、31.38%和138.63%；与同剂量的玉米肽相比，玉米糖肽具有更显著的抗运动性疲劳效果。综上，酶法糖基化修饰能够显著提高玉米肽的抗疲劳活性，其机制可能与缓解氧化性损伤、降低体内积聚的乳酸含量和增加肌肉和肝脏中糖原含量有关。 |
| 英文摘要: |
| In order to provide a theoretical basis for the development of anti-fatigue products of zein glycopeptides, zein glycopeptides were prepared by enzymatic glycosylation of zein peptides (prepared by Alcalase-hydrolyzed zein) with transglutaminase as catalyst and D-glucosamine as acyl receptor. Zein glycopeptides were used as the test samples(low, medium and high doses of 125, 250 mg/kg and 500 mg/kg, respectively), with zein peptides as the negative control (dose of 500 mg/kg). After 28 d of continuous gavage administration, the body weight, exercise endurance and fatigue biochemical indexes of mice were detected to evaluate the effects of zein glycopeptides on growth of mice and their anti-fatigue effects. The results showed that the experimental dose of zein glycopeptides and zein peptides had no adverse effects on the growth of mice. Compared with the normal group (gavaged with normal saline), 250 mg/kg zein glycopeptides significantly prolonged the rotarod test time and the weight-located swimming time to 5.71 times and 2.48 times, respectively, while the zein peptides group showed no significant changes in these parameters. Compared with the model group, 250 mg/kg zein glycopeptides decreased the activity of lactate dehydrogenase and the contents of serum lactic acid, serum urea nitrogen and liver malondialdehyde by 30.29%, 1320%, 31.59% and 50.59%, respectively, while increased the contents of liver glycogen, muscle glycogen and glutathione, and the activities of superoxide dismutase and glutathione peroxidase by 18.38%, 54.40%, 42.20%, 31.38% and 138.63%, respectively. Compared with the same dose of zein peptides, zein glycopeptides had a more significant effect on anti-exercise fatigue. In conclusion, enzymatic glycosylation can significantly improve the anti-fatigue activity of zein peptides, which might be related to alleviate oxidative damage, reduce the accumulated lactic acid content in the body and increase the glycogen content in muscle and liver. |
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