| 李士鹏1,2，綦文涛2，彭文婷2，刘兴泉1，郭伟群1,2.发酵前包被裂殖壶菌微胶囊工艺优化及其对
裂殖壶菌生长和代谢特性的影响[J].中国油脂,2025,50(5):.[LI Shipeng1,2, QI Wentao2, PENG Wenting2, LIU Xingquan1, GUO Weiqun1,2.Optimization of the preparation process of Schizochytrium sp. microcapsules before fermentation and its effect on the growth and metabolic properties of Schizochytrium sp.[J].China Oils and Fats,2025,50(5):.] |
| 发酵前包被裂殖壶菌微胶囊工艺优化及其对
裂殖壶菌生长和代谢特性的影响 |
| Optimization of the preparation process of Schizochytrium sp. microcapsules before fermentation and its effect on the growth and metabolic properties of Schizochytrium sp. |
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| DOI：10.19902/j.cnki.zgyz.1003-7969.240116 |
| 中文关键词: 乳化凝胶法 发酵前包被微胶囊 二十二碳六烯酸 裂殖壶菌 生长 代谢 耐热性 |
| 英文关键词:emulsified gel method encapsulation before fermentation docosahexaenoic acid Schizochytrium sp. growth metabolism heat resistance |
| 基金项目:中央级公益性科研院所基本科研业务费专项课题（ZX2214） |
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| Author Name | Affiliation | | LI Shipeng1,2, QI Wentao2, PENG Wenting2, LIU Xingquan1, GUO Weiqun1,2 | 1.College of Food and Health,Zhejiang A & F University, Hangzhou 311300, China； 2.Institute of Food Quality and Nutrition, Academy of National Food and Strategic Reserves Administration, Bejing 100037, China |
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| 中文摘要: |
| 为了探究发酵前微胶囊化对裂殖壶菌生长代谢及二十二碳六烯酸（DHA）生产的影响，采用内源乳化凝胶法制备发酵前裂殖壶菌微胶囊，通过单因素实验和正交实验优化微胶囊制备工艺，并探究了裂殖壶菌在固化核心和液化核心两种微胶囊条件下的生长、代谢特性及耐热性。结果表明：裂殖壶菌微胶囊的最佳制备条件为海藻酸钠质量浓度12 g/L、Span 85添加量2 g/L、水油相体积比1∶ 3、搅拌速度300 r/min，在此条件下微胶囊平均粒径为（420.49±23.12） μm，最适粒径比例为55.74%，得率为73.10%。微胶囊环境下裂殖壶菌呈现聚团生长特征；与游离培养相比，微胶囊培养裂殖壶菌的葡萄糖消耗无显著变化，而pH变化和氮源消耗显著变慢；与游离培养相比，裂殖壶菌在液化核心微胶囊条件下生物量、油脂含量和DHA含量没有明显变化，而在固化核心微胶囊条件下均显著下降；微胶囊化提高了裂殖壶菌高温下的耐热性和胞内DHA保存率。综上，发酵前包被微胶囊有助于裂殖壶菌耐热性的提高，且液化核心微胶囊更有利于裂殖壶菌的生长、代谢。 |
| 英文摘要: |
| Optimization of the preparation process of Schizochytrium sp. microcapsules
before fermentation and its effect on the growth and metabolic
properties of Schizochytrium sp. |
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