| 章敏，祝洪刚，俞春莲.山茶油基甘油二酯的酶解制备工艺优化[J].中国油脂,2025,50(9):.[ZHANG Min, ZHU Honggang, YU Chunlian.Optimization of enzymatic hydrolysis preparation process for camellia seed oil based diacylglycerol[J].China Oils and Fats,2025,50(9):.] |
| 山茶油基甘油二酯的酶解制备工艺优化 |
| Optimization of enzymatic hydrolysis preparation process for camellia seed oil based diacylglycerol |
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| DOI：10.19902/j.cnki.zgyz.1003-7969.240422 |
| 中文关键词: 甘油二酯 山茶油 固定化脂肪酶 甘油解 |
| 英文关键词:diacylglycerol camellia seed oil immobilized lipase glycerol hydrolysis |
| 基金项目:浙江省重点——科技强农专项（2023K099） |
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| 中文摘要: |
| 旨在为山茶油的利用奠定基础，以精炼山茶油和甘油为底物，在无溶剂体系中通过酶法催化甘油解反应制备DAG。考察了固定化脂肪酶种类、反应温度、加酶量、反应时间、底物物质的量比及初始加水量对产物中DAG含量的影响，在此基础上，通过响应面实验优化山茶油基DAG的酶解制备工艺，并考察了固定化脂肪酶的重复使用性。结果表明：山茶油基DAG的最佳酶解制备工艺条件为采用固定化脂肪酶Lipozyme 435为催化剂、山茶油与甘油物质的量比1∶ 3、初始加水量1.5%（以底物总质量计）、加酶量6.5%（以底物总质量计）、反应温度69 ℃、反应时间11 h，在此条件下产物中DAG含量达到52.83%（1,3-DAG含量42.38%，1,2-DAG含量10.45%）。固定化脂肪酶循环使用5次后，产物中DAG含量无明显降低，其相对酶活力依然维持在90%左右。综上，采用固定化脂肪酶催化山茶油甘油解制备的山茶油基DAG中DAG含量高，该工艺适合用于山茶油的深度加工。 |
| 英文摘要: |
| To lay the foundation for the utilization of camellia seed oil based diacylglycerol (DAG), DAG was prepared by enzymatic catalysis of glycerol hydrolysis reaction in a solvent-free system using refined camellia seed oil and glycerol as substrates. The effects of immobilized lipase type, reaction temperature, enzyme dosage, reaction time, substrate molar ratio, and initial water dosage on the DAG content in the product were investigated. On this basis, the enzymatic hydrolysis preparation process of camellia seed oil based DAG was optimized by response surface methodology. The reusability of immobilized lipase was also examined. The results showed that the optimal enzymatic hydrolysis conditions for preparing camellia seed oil based DAG were obtained as follows: using immobilized lipase Lipozyme 435 as the catalyst, molar ratio of camellia seed oil to glycerol 1∶ 3, initial water dosage 1.5%(based on total mass of substrate), enzyme dosage 6.5%(based on total mass of substrate), reaction temperature 69 ℃, and reaction time 11 h. Under these conditions, the DAG content in the product reached 52.83% (1,3-DAG content of 42.38%, 1,2-DAG content of 10.45%). The DAG content of the immobilized lipase did not decrease significantly after 5 cycles, and its relative enzyme activity was still maintained at about 90%. In summary, the camellia seed oil based DAG prepared by immobilized lipase catalyzed glycerol hydrolysis of camellia seed oil has a high content of DAG and the process is suitable for deep processing of camellia seed oil. |
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