| 刘小玉1,2， 曹红星1,2， 李欣瑜1,2， 周丽霞1,2， 李睿1,2， 李启黉1,2， 付登强1,2.无籽种油棕果实发育中脂质合成转录代谢差异分析[J].中国油脂,2025,50(11):.[LIU Xiaoyu1,2, CAO Hongxing1,2, LI Xinyu1,2, ZHOU Lixia1,2, LI Rui1,2,
LI Qihong1,2, FU Dengqiang1,2.Differential transcriptomic and metabolomic analysis of lipid synthesis during seedless oil palm fruit development[J].China Oils and Fats,2025,50(11):.] |
| 无籽种油棕果实发育中脂质合成转录代谢差异分析 |
| Differential transcriptomic and metabolomic analysis of lipid synthesis during seedless oil palm fruit development |
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出版日期：2025-11-20
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| DOI：10.19902/j.cnki.zgyz.1003-7969.240465 |
| 中文关键词: 油棕 果实发育 脂质 代谢组学 转录组学 |
| 英文关键词:oil palm fruit development lipid metabolomics transcriptomics |
| 基金项目:海南省国际科技合作研发项目（GHYF2024019）；热带作物生物育种全国重点实验室开放课题项目(SKLTCBYWF202502) |
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| Author Name | Affiliation | | LIU Xiaoyu1,2, CAO Hongxing1,2, LI Xinyu1,2, ZHOU Lixia1,2, LI Rui1,2,
LI Qihong1,2, FU Dengqiang1,2 | 1.Coconut Research Institute, Chinese Academy of Tropical Agricultural Science,
Wenchang 571339, Hainan, China
2.Hainan Key Laboratory of Tropical Oil
Crops Biology,Wenchang 571339, Hainan, China |
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| 摘要点击次数: 1395 |
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| 中文摘要: |
| 为揭示无籽种油棕果实中脂质代谢物合成的调控机制，以授粉后95、125、185 d不同发育时期的无籽种油棕果实为试验材料，采用液相色谱-质谱联用（LC-MS/MS）和RNA-Seq技术对其脂质合成代谢组和转录组变化进行测定，分析无籽种油棕果实发育过程中脂质合成转录代谢的差异。结果表明：在无籽种油棕果实发育过程中共鉴定出5个脂质大类、26个脂质亚类，且随着油棕果实的成熟，甘油磷脂类逐渐转化为甘油酯类和脂肪酰类；代谢组和转录组联合分析表明，PLA2、SDR表达量与神经酰胺、磷脂酰胆碱、磷脂酰乙醇胺、磷脂酰甘油呈极显著正相关，与甘油二酯、甘油三酯呈极显著负相关，而ACSL4表达量与神经酰胺、磷脂酰胆碱、磷脂酰乙醇胺、磷脂酰甘油呈极显著负相关，与甘油二酯、甘油三酯呈极显著正相关。综上，无籽种油棕果实发育过程中，PLA2、SDR可能是调控鞘脂类和甘油磷脂类合成的关键基因，ACSL4可能是调控甘油酯类合成的关键基因。 |
| 英文摘要: |
| In order to reveal the regulatory mechanism of lipid metabolite synthesis in seedless oil palm fruits, with seedless oil palm fruits at 95, 125 d and 185 d after pollination as raw materials, the changes of its transcriptome and metabolome of lipid synthesis were determined by LC-MS/MS and RNA-Seq techniques, and the difference in transcriptomics and metabolomics of lipid synthesis during seedless oil palm fruit development was analyzed. The results showed that 5 lipid groups and 26 lipid subclasses were identified during the fruit development. As the oil palm fruit matures, glycerophospholipids gradually transformed into glycerolipids and fatty acyls. The results of joint analysis of transcriptome and metabolome showed that the expression levels of PLA2 and SDR were highly positively correlated with ceramide, phosphatidylcholine, phosphatidylethanolamine and phosphatidylglycerol, and highly negatively correlated with diglyceride and triglyceride, while the expression level of ACSL4 was highly negatively correlated with ceramide, phosphatidylcholine, phosphatidylethanolamine and phosphatidylglycerol, and highly positively correlated with diglyceride and triglyceride. In conclusion, PLA2 and SDR may be the key genes regulating the synthesis of sphingolipids and glycerophospholipids, and ACSL4 may be the key gene regulating the synthesis of glycerolipids during seedless oil palm fruit development. |
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