| 杜舒梦1，李蕊1，刘元法1,2，杨巍1,2，叶展1,2.3种典型不饱和脂肪酸对Caco-2肠上皮细胞
机械屏障功能的影响[J].中国油脂,2025,50(12):.[DU Shumeng1, LI Rui1, LIU Yuanfa1,2, YANG Wei1,2, YE Zhan1,2.Influences of three typical unsaturated fatty acids on mechanical barrier function of Caco-2 intestinal epithelial cells[J].China Oils and Fats,2025,50(12):.] |
| 3种典型不饱和脂肪酸对Caco-2肠上皮细胞
机械屏障功能的影响 |
| Influences of three typical unsaturated fatty acids on mechanical barrier function of Caco-2 intestinal epithelial cells |
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| DOI：10.19902/j.cnki.zgyz.1003-7969.240682 |
| 中文关键词: 不饱和脂肪酸 肠道机械屏障 Caco-2细胞模型 营养特性 |
| 英文关键词:unsaturated fatty acids intestinal mechanical barrier Caco-2 cell model nutritional attributes |
| 基金项目:“十四五”国家重点研发计划（2023YFD2100400）；国家自然科学基金青年基金（32201936）；江苏省自然科学基金青年基金（BK20221076） |
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| Author Name | Affiliation | | DU Shumeng1, LI Rui1, LIU Yuanfa1,2, YANG Wei1,2, YE Zhan1,2 | 1.School of Food Science and Technology, Jiangnan University, Wuxi 214122, Jiangsu, China
2.State Key Laboratory of Food Science and Resources, Wuxi 214122, Jiangsu, China |
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| 中文摘要: |
| 旨在为优化膳食脂肪摄入结构，预防相关慢性代谢类疾病提供一定的理论依据，通过构建Caco-2肠上皮细胞机械屏障模型，考察不同脂肪酸〔油酸（OA）、亚油酸（LA）和α-亚麻酸（ALA）〕对Caco-2细胞模型完整性和通透性的影响，以及对Caco-2细胞机械屏障相关基因及关键蛋白表达水平的调控作用。结果表明：在脂肪酸浓度300 μmol/L、干预时间24 h条件下，LA显著降低跨膜电阻值和碱性磷酸酶比活性，升高表观渗透系数和一氧化氮含量，而OA和ALA对上述指标无显著影响；LA显著下调胞质紧密黏连蛋白1（ZO1）基因和闭锁蛋白（OCLN）基因表达水平，对封闭蛋白2（CLDN2）基因的表达无显著影响；ALA显著提高ZO1基因的表达水平并抑制CLDN2基因的表达水平，而OA对ZO1、OCLN、CLDN2基因的表达水平无显著影响；3种脂肪酸对屏障黏附连接蛋白和桥粒蛋白相关基因的表达均无显著影响；LA显著降低胞质紧密黏进蛋白1（ZO-1）和闭锁蛋白（Occludin）的表达水平，ALA显著降低封闭蛋白2（Claudin-2）的表达水平，OA对紧密连接蛋白的表达水平无显著影响。综上，3种典型不饱和脂肪酸对Caco-2细胞屏障功能影响不同，LA可通过降低紧密连接蛋白表达水平来降低肠屏障的完整性，增加其通透性，而ALA则表现出提升肠屏障紧密性的效果，OA则未见明显影响。 |
| 英文摘要: |
| In order to provide a theoretical basis for optimizing dietary fat intake structure and preventing related chronic metabolic diseases, by constructing a Caco-2 intestinal epithelial cell mechanical barrier model, the effects of different fatty acids (oleic acid (OA), linoleic acid (LA), and α-linolenic acid (ALA)) on the integrity and permeability of the Caco-2 cell model were investigated, as well as their regulatory effects on the expression levels of genes and key proteins related to the mechanical barrier of Caco-2 cells. The results showed that under the conditions of fatty acid concentration 300 μmol/L and intervention time 24 h, LA significantly reduced the transmembrane resistance value and alkaline phosphatase specific activity, increased the apparent osmotic coefficient and nitric oxide content, while OA and ALA had no significant effect on the above indicators. LA significantly downregulated the expression levels of the tight junction protein 1 (ZO1) gene and the occludin (OCLN) gene, but had no significant effect on the expression of the claudin 2 (CLDN2) gene. ALA significantly increased the expression level of the ZO1 gene and inhibited the expression level of the CLDN2 gene, while OA had no significant effect on the expression levels of the ZO1, OCLN, and CLDN2 genes. The three fatty acids had no significant effects on the expression of barrier adhesion junction protein and desmosome protein-related genes. LA significantly reduced the expression levels of tight junction protein 1(ZO-1) and Occludin, ALA significantly reduced the expression level of Claudin-2, and OA had no significant effect on the expression levels of tight junction proteins. In conclusion, the three typical unsaturated fatty acids have different effects on the barrier function of Caco-2 cells. LA can reduce the integrity of the intestinal barrier and increase its permeability by lowering the expression level of tight junction proteins, ALA promotes the tightness of the intestinal barrier, while OA has no obvious influence. |
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