成文荷1,2,王小妹1,2,王宁丽2,裴栋2,3,刘宝乾2,邸多隆1,2.油橄榄叶中活性成分含量测定及抗氧化能力研究[J].中国油脂,2025,50(12):.[CHENG Wenhe1,2, WANG Xiaomei1,2, WANG Ningli2, PEI Dong2,3, LIU Baoqian2, DI Duolong1,2.Determination of active ingredients contents and antioxidant capacity of olive leaves[J].China Oils and Fats,2025,50(12):.]
油橄榄叶中活性成分含量测定及抗氧化能力研究
Determination of active ingredients contents and antioxidant capacity of olive leaves
  
DOI:10.19902/j.cnki.zgyz.1003-7969.240557
中文关键词:  油橄榄叶  木犀草苷  橄榄苦苷  抗氧化能力
英文关键词:olive leaf  luteoloside  oleuropein  antioxidant capacity
基金项目:云南省重大科技专项计划(202302AE090007);云南省重点研发计划(202203AD150003);陇南市科技计划项目(2023-S·QKJ-02)
作者单位
成文荷1,2,王小妹1,2,王宁丽2,裴栋2,3,刘宝乾2,邸多隆1,2 1.甘肃中医药大学 药学院兰州 730000 2.中国科学院兰州化学物理研究所中国科学院 西北特色植物资源化学重点实验室甘肃省天然药物重点实验室兰州 730000 3.云南油橄榄大健康产业创新研究发展有限公司云南 丽江 674100 
Author NameAffiliation
CHENG Wenhe1,2, WANG Xiaomei1,2, WANG Ningli2, PEI Dong2,3, LIU Baoqian2, DI Duolong1,2 1.College of Pharmacy, Gansu University of Chinese Medicine, Lanzhou 730000, China
2.Key Laboratory for Natural Medicine of Gansu Province,CAS Key Laboratory of Chemistry of Northwestern Plant Resources, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, China
3.Yunnan Olive Health Industry Innovation Research and Development Co. , Ltd. , Lijiang 674100, Yunnan,China 
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中文摘要:
      旨在为油橄榄叶在食品、保健品、药品等领域的应用提供参考,以不同品种、产地和采收时间的32批油橄榄叶为原料,测定其中的木犀草苷和橄榄苦苷含量及油橄榄叶的抗氧化能力(DPPH自由基清除能力、ABTS自由基清除能力、总还原能力),并对油橄榄叶的木犀草苷和橄榄苦苷含量与油橄榄叶抗氧化能力进行了相关性分析和聚类分析。结果表明:不同油橄榄叶中的橄榄苦苷含量普遍高于木犀草苷含量;品种、产地和采收时间对油橄榄叶中的木犀草苷含量和橄榄苦苷含量均有影响,其中对橄榄苦苷含量的影响较大。油橄榄叶具有一定的DPPH自由基清除能力、ABTS自由基清除能力、总还原能力,且不同品种、产地和采收时间的油橄榄叶总还原能力总体差异不大。相关性分析和聚类分析结果显示,油橄榄叶中橄榄苦苷、木犀草苷含量均与其抗氧化能力呈极显著正相关,且整体来看,油橄榄叶中橄榄苦苷含量与抗氧化能力的相关性更强。综上,橄榄苦苷是油橄榄叶抗氧化能力的主要贡献成分,可作为质量控制的关键指标,同时木犀草苷的协同作用也极为重要。
英文摘要:
      To provide a reference for the application of olive leaves in food, dietary supplements, and pharmaceuticals, 32 batches of olive leaves from different cultivars, origins, and harvest time were analyzed for their luteoloside and oleuropein contents, as well as for its antioxidant capacity(DPPH radical scavenging activity, ABTS radical scavenging activity, and total reducing capacity). Correlation and cluster analysis were further performed to examine the relationships between the luteoloside and oleuropein contents and the antioxidant capacity. The results showed that oleuropein was consistently more abundant than luteoloside in all olive leaf samples. Both cultivar and origin, along with harvest time, influenced the contents of the two compounds, with oleuropein exhibiting the larger variation. All olive leaves exhibited notable DPPH and ABTS radical scavenging activities as well as total reducing capacity, although the overall variation in total reducing capacity among different samples was relatively small. Correlation and cluster analysis revealed that both oleuropein and luteoloside contents were significantly positively correlated with antioxidant capacity, with oleuropein demonstrating the stronger association. In conclusion, oleuropein is the principal contributor to the antioxidant capacity of olive leaves and can serve as a key quality-control marker, while luteoloside provides an important synergistic effect.
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