| 崔宁1，李金林1，胡明明1，涂宗财1,2，陈云云3，彭斌1.4种来源鱿鱼内脏油中甘油三酯和甘油磷脂的
脂肪酸组成及位置分布[J].中国油脂,2025,50(12):.[CUI Ning1, LI Jinlin1, HU Mingming1, TU Zongcai1,2,
CHEN Yunyun3, PENG Bin1.Composition and positional distribution of fatty acids in triacylglycerols and glycerophospholipids from the viscera oil of four sources squids[J].China Oils and Fats,2025,50(12):.] |
| 4种来源鱿鱼内脏油中甘油三酯和甘油磷脂的
脂肪酸组成及位置分布 |
| Composition and positional distribution of fatty acids in triacylglycerols and glycerophospholipids from the viscera oil of four sources squids |
| |
| DOI：10.19902/j.cnki.zgyz.1003-7969.240626 |
| 中文关键词: 鱿鱼内脏 鱼油 脂肪酸 位置分布 |
| 英文关键词:squid viscera fish oil fatty acid positional distribution |
| 基金项目:江西省自然科学基金青年基金项目（20232BAB215060）；国家自然科学基金项目（32060557）；2023年度浙江省博士后科研项目择优资助（319030） |
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| Author Name | Affiliation | | CUI Ning1, LI Jinlin1, HU Mingming1, TU Zongcai1,2,
CHEN Yunyun3, PENG Bin1 | 1.National R&D Branch Center for Conventional Freshwater Fish Processing, College of Life Science, Jiangxi
Normal University, Nanchang 330022, China
2.State Key Laboratory of Food Science and Resources,
Nanchang University, Nanchang 330047, China
3.China Aquatic Products Zhoushan Marine Fisheries
Corporation, Zhoushan 316000, Zhejiang, China |
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| 中文摘要: |
| 为挖掘鱿鱼内脏脂质的营养价值，采用超声辅助溶剂法提取4种不同来源〔阿根廷、北太平洋（北太）、赤道和秘鲁〕鱿鱼内脏中油脂，采用固相萃取、专一性酶解和气相色谱-质谱联用（GC-MS）等技术，分析不同鱿鱼内脏油甘油三酯和甘油磷脂的脂肪酸组成及其在甘油骨架上位置分布。结果表明：4种鱿鱼内脏油甘油三酯中共检出42种脂肪酸，包括18种饱和脂肪酸（SFA）、9种单不饱和脂肪酸（MUFA）和15种多不饱和脂肪酸（PUFA）,SFA、MUFA和PUFA在甘油三酯中的含量分别为31.79%~33.24%、32.99%~35.02%和33.19%~33.90%；秘鲁和赤道鱿鱼内脏油甘油三酯的SFA含量较高，分别为33.24%和33.11%，显著高于阿根廷与北太鱿鱼（p<0.05）；赤道与秘鲁鱿鱼内脏油甘油三酯的MUFA含量无显著差异（p>0.05），但显著低于阿根廷和北太鱿鱼（p<005）；赤道、阿根廷和秘鲁鱿鱼内脏油甘油三酯的PUFA含量无显著差异（p>005），显著高于北太鱿鱼（p<0.05）；MUFA主要分布在甘油三酯的Sn-2位（39.78%~4198%），而SFA和PUFA更易分布在Sn-1,3位，含量分别为32.84%~34.15%和35.62%~3634%。4种鱿鱼内脏油甘油磷脂中共检出83种脂肪酸，含21种SFA、19种MUFA和43种PUFA；甘油磷脂上3类脂肪酸含量高低顺序为PUFA>SFA>MUFA；阿根廷和北太鱿鱼内脏油甘油磷脂的PUFA含量显著高于赤道与秘鲁鱿鱼（p<0.05）；甘油磷脂的Sn-1位分布的脂肪酸主要为SFA和MUFA，含量分别为40.60%~40.91%和25.10%~25.28%，而Sn-2位分布的脂肪酸主要为PUFA（6995%~70.10%）。综上，4种不同来源鱿鱼内脏油的甘油三酯和甘油磷脂在脂肪酸组成上存在显著差异，且不同脂肪酸在甘油骨架上的酰化位置具有选择性。 |
| 英文摘要: |
| To explore the nutritional value of squid visceral lipids, oils were extracted from the viscera of four sources squids(Argentine, North Pacific, Equatorial and Peruvian) using ultrasound-assisted solvent extraction. The fatty acid composition of triacylglycerols and glycerophospholipids in squid visceral oils, as well as their positional distribution on the glycerol backbone, were analyzed by solid-phase extraction (SPE), regioselective enzymatic hydrolysis, and gas chromatography-mass spectrometry (GC-MS). The results showed that a total of 42 fatty acids were identified in triacylglycerols from the viscera oil of four squids, including 18 saturated fatty acids (SFA), 9 monounsaturated fatty acids (MUFA), and 15 polyunsaturated fatty acids (PUFA). The relative contents of SFA, MUFA, and PUFA in triacylglycerols were 31.79%-33.24%, 32.99%-3502%, and 33.19%-33.90%, respectively. The triacylglycerols from Peruvian, and Equatorial squid viscera oil showed the highest SFA contents (33.24% and 33.11%, respectively), which were significantly higher than those from Argentine and North Pacific squids (p<0.05). There was no significant difference in MUFA content of triacylglycerols of viscera oil between Equatorial and Peruvian squids(p>0.05), but their levels were significantly lower than those from Argentine and North Pacific squids (p<0.05). Regarding PUFA, no significant differences were found among Equatorial, Argentine, and Peruvian squids (p>005), but their levels were significantly higher than that in North Pacific squid (p<0.05). MUFA were predominantly esterified at the Sn-2 position of triacylglycerols (39.78%-41.98%), while SFA and PUFA showed preferential localization at the Sn-1,3 positions, accounting for 32.84%-34.15% and 35.62%-36.34% of total fatty acids, respectively. A total of 83 fatty acids were identified in glycerophospholipids, comprising 21 SFA, 19 MUFA, and 43 PUFA. The relative contents of these fatty acid classes in glycerophospholipids followed the hierarchical order: PUFA>SFA>MUFA. The contents of PUFA in visceral glycerophospholipids in Argentine and North Pacific squids were significantly higher than those in Equatorial and Peruvian squids (p<0.05). The fatty acids at the Sn-1 position of the glycerophospholipids were primarily SFA and MUFA, accounting for 40.60%-40.91% and 25.10%-25.28%, respectively. In contrast, the Sn-2 position was predominantly occupied by PUFA (69.95%-70.10%). In conclusion, triacylglycerols and glycerophospholipids of squid viscera oils from four different sources differ significantly in fatty acid composition, and the acylation positions of different fatty acids on the glycerol backbone are selective. |
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