| 周原1,2，董曼1,2，刘坤1,2，李宏铨1,2，何东平2,3，高盼2,3，
王澍1,2，康翠欣1,2，何艳1,2，王煜红1,2.高效液相色谱法快速同时测定芝麻油
及芝麻调和油中8种特征香味物质[J].中国油脂,2025,50(12):.[ZHOU Yuan1,2, DONG Man1,2, LIU Kun1,2, LI Hongquan1,2, HE Dongping2,3,
GAO Pan2,3, WANG Shu1,2, KANG Cuixin1,2, HE Yan1,2, WANG Yuhong1,2.Rapid and simultaneous determination of 8 characteristic aroma compounds in sesame oil and sesame blended oil by HPLC[J].China Oils and Fats,2025,50(12):.] |
| 高效液相色谱法快速同时测定芝麻油
及芝麻调和油中8种特征香味物质 |
| Rapid and simultaneous determination of 8 characteristic aroma compounds in sesame oil and sesame blended oil by HPLC |
| |
| DOI：10.19902/j.cnki.zgyz.1003-7969.240564 |
| 中文关键词: 高效液相色谱 芝麻油 芝麻调和油 特征香味物质 |
| 英文关键词:high performance liquid chromatography sesame oil sesame blended oil characteristic aroma compounds |
| 基金项目:国家市场监督管理总局科技计划项目（2022MK150） |
| 作者 | 单位 | | 周原1,2，董曼1,2，刘坤1,2，李宏铨1,2，何东平2,3，高盼2,3，
王澍1,2，康翠欣1,2，何艳1,2，王煜红1,2 | 1.武汉食品化妆品检验所，武汉 430040； 2.国家市场监督管理总局重点实验室（食用油质量与安全），
武汉 430040； 3.武汉轻工大学 食品科学与工程学院，武汉 430023 |
|
| Author Name | Affiliation | | ZHOU Yuan1,2, DONG Man1,2, LIU Kun1,2, LI Hongquan1,2, HE Dongping2,3,
GAO Pan2,3, WANG Shu1,2, KANG Cuixin1,2, HE Yan1,2, WANG Yuhong1,2 | 1.Wuhan Institute for Food and Cosmetic Control, Wuhan 430040, China
2.Key Laboratory of Edible
Oil Quality and Safety, State Administration for Market Regulation, Wuhan 430040, China
3.College of Food Science and Engineering, Wuhan Polytechnic University,
Wuhan 430023, China |
|
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| 中文摘要: |
| 旨在为芝麻油市场监管提供技术支撑，建立了一种基于高效液相色谱技术同时测定芝麻油及芝麻调和油中8种特征香味物质含量的方法。样品(5 g)用10 mL甲醇超声提取20 min,经Phenomenex Gemini C6-Phenyl 110 色谱柱(250 mm×4.6 mm，5 μm)分离，柱温35 ℃，以乙腈-0.1%磷酸水溶液（体积比12∶ 88）为流动相进行等度洗脱，采用配备二极管阵列检测器的高效液相色谱仪进行检测，外标法定量。方法学考察结果表明，8种特征香味物质在0.5～10.0 μg/mL质量浓度范围内线性关系良好，决定系数超过0.999，方法的检出限和定量限分别为0.4 mg/kg和1.0 mg/kg，加标回收率为84.00%～109.00%，精密度相对标准偏差为0.19%～1.68%。综上，该方法具有较高的灵敏度和精密度，能够准确、快速地同时检测芝麻油及芝麻调和油中8种特征香味物质。 |
| 英文摘要: |
| In order to provide technical support for the market supervision of sesame oil, a method based on high performance liquid chromatography was established for the simultaneous determination of 8 characteristic aroma compounds in sesame oil and sesame blended oil. The samples (5 g) were extracted with 10 mL of methanol and ultrasonically extracted for 20 min. The extract were separated on a Phenomenex Gemini C6-Phenyl 110 column (250 mm×4.6 mm, 5 μm), column temperature at 35 ℃. The mobile phase was acetonitrile-0.1% phosphoric acid aqueous solution (volume ratio 12∶ 88) for isocratic elution. Detection was carried out using a high performance liquid chromatography with diode array detector and external standard quantification. The results of the methodological investigation showed that the 8 characteristic aroma compounds had a good linear relationship within the mass concentration range of 0.5-10.0 μg/mL, with determination coefficient exceeding 0.999. The detection limit and quantification limit of the method were 0.4 mg/kg and 1.0 mg/kg, respectively. The spiked recovery was 84.00%-109.00%, and the relative standard deviation for precision was 0.19%-1.68%. In summary, this method has high sensitivity and precision, and can accurately and quickly detect 8 characteristic aroma compounds in sesame oil and sesame blended oil simultaneously. |
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