| 李良萌1，石亚楠2，洪永德2，陈明1，付冬梅1.用于制备牛油果甘油二酯油的脂肪酶固定化研究[J].中国油脂,2025,50(12):.[LI Liangmeng1, SHI Yanan2, HONG Yongde2, CHEN Ming1, FU Dongmei1.Immobilization of lipase for preparation of avocado diacylglycerol oil[J].China Oils and Fats,2025,50(12):.] |
| 用于制备牛油果甘油二酯油的脂肪酶固定化研究 |
| Immobilization of lipase for preparation of avocado diacylglycerol oil |
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| DOI：10.19902/j.cnki.zgyz.1003-7969.240612 |
| 中文关键词: 固定化脂肪酶 响应面法 牛油果油 甘油二酯 |
| 英文关键词:immobilized lipase response surface methodology avocado oil diacylglycerol |
| 基金项目:十四五”国家重点研发计划项目（2023YFF1103505）；2021年辽宁省首批“揭榜挂帅”科技攻关项目（2021JH1/10400057） |
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| Author Name | Affiliation | | LI Liangmeng1, SHI Yanan2, HONG Yongde2, CHEN Ming1, FU Dongmei1 | 1.School of Biological Engineering, Dalian Polytechnic University, Dalian 116034, Liaoning, China
2.INNOBIO Corporation Limited, Dalian 116600, Liaoning, China |
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| 中文摘要: |
| 旨在通过固定化技术提高脂肪酶的可重复利用性，推动功能性油脂的健康应用。利用大孔吸附树脂对脂肪酶固定化，并通过单因素实验和响应面法优化固定化条件，然后将固定化脂肪酶应用于牛油果甘油二酯油的制备，并分析牛油果甘油二酯油的脂肪酸组成，最后对固定化脂肪酶的重复使用性和储藏稳定性进行分析。结果表明，最佳的脂肪酶固定化条件为以大孔吸附树脂D101为载体、脂肪酶质量浓度0.21 g/mL（10 g载体）、固定化时间5 h、固定化温度41 ℃、磷酸盐缓冲溶液pH 7.5，在此条件下得到的固定化脂肪酶的酶活为6.871 U/mg。固定化脂肪酶催化制备的牛油果甘油二酯油的甘油二酯含量可达81.42%，且其脂肪酸组成与牛油果油基本一致。固定化脂肪酶连续使用6次后，酶活为初始酶活的62.2%；4 ℃下储藏30 d，固定化脂肪酶的酶活保持在初始酶活的92.1%。综上，通过吸附法制备固定化脂肪酶可应用于牛油果甘油二酯油的制备，且不改变牛油果油原有的脂肪酸组成。 |
| 英文摘要: |
| The aim is to enhance the reusability of lipase, thereby promoting the application of functional lipids.The lipase was immobilized on macroporous resin, and the immobilization conditions were optimized through single-factor experiments and response surface methodology. The prepared immobilized lipase was applied to the preparation of avocado diacylglycerol oil, and the fatty acid composition of avocado diacylglycerol oil was analyzed, and its reusability and storage stability were analyzed. The results showed that the optimal immobilization conditions for the lipase were macroporous resin D101 as the carrier, lipase mass concentration 0.21 g/mL (10 g carrier), immobilization time 5 h, immobilization temperature 41 ℃, and phosphate buffer solution pH 7.5. Under these conditions, the enzyme activity of the immobilized lipase was 6.871 U/mg. The immobilized lipase was used to prepare avocado diacylglycerol oil, and the content of diacylglycerol reached 81.42%. Moreover, the fatty acid composition of avocado diacylglycerol oil and avocado oil were consistent. The enzyme activity remained at 62.2% of the initial activity after 6 times consecutive uses, and 92.1% of the initial activity after 30 d of storage at 4 ℃. In conclusion, the immobilized lipase prepared by adsorption can be applied in the production of avocado diacylglycerol oil without altering the original fatty acid composition of avocado oil. |
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