| 伍晓然1，王湘林1, 2，刘翔燕1，郑泽杰1，曹潇玉1，何俊杰1，文利新1，王吉1.猪油、油茶籽油和花生油对小鼠糖脂代谢的影响[J].中国油脂,2026,51(1):.[WU Xiaoran1, WANG Xianglin1,2, LIU Xiangyan1, ZHENG Zejie1,
CAO Xiaoyu1, HE Junjie1, WEN Lixin1, WANG Ji1.Effects of lard, camellia seed oil and peanut oil on glucose and lipid metabolism in mice[J].China Oils and Fats,2026,51(1):.] |
| 猪油、油茶籽油和花生油对小鼠糖脂代谢的影响 |
| Effects of lard, camellia seed oil and peanut oil on glucose and lipid metabolism in mice |
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| DOI：10.19902/j.cnki.zgyz.1003-7969.240526 |
| 中文关键词: 食用油 花生油 油茶籽油 猪油 糖脂代谢 |
| 英文关键词:edible oil peanut oil camellia seed oil lard glucose and lipid metabolism |
| 基金项目:国家自然科学基金面上项目(32373079)；校企横向课题(2019xny-js044) |
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| Author Name | Affiliation | | WU Xiaoran1, WANG Xianglin1,2, LIU Xiangyan1, ZHENG Zejie1,
CAO Xiaoyu1, HE Junjie1, WEN Lixin1, WANG Ji1 | (1.Hunan Engineering Research Center of Livestock and Poultry Health Care, College of Veterinary
Medicine, Hunan Agricultural University, Changsha 410128, China
2.Changsha Lvye
Biotechnology Co. , Ltd. , Changsha 410100, China) |
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| 中文摘要: |
| 旨在探究常用食用油及目前实际油脂供能水平对人体糖脂代谢的影响，为我国居民油脂选择提供参考，将54只C57BL/6J小鼠平均分为6组，即低脂饮食（15%脂肪供能水平，对照饮食）和高脂饮食（35%脂肪供能水平，模拟我国居民饮食实际油脂供能水平）的猪油组、油茶籽油组和花生油组，饲养24周，每周记录小鼠体质量，分别于22周和23周时测定小鼠葡萄糖耐量和胰岛素耐量，饲养结束后检测小鼠空腹血糖（FBG）水平、体脂率、血脂水平（TC、TG、LDL-C、HDL-C），并对小鼠肝脏病理学，肝脏TC、TG水平，血清谷丙转氨酶（ALT）、谷草转氨酶（AST）活性，以及肝脏糖脂代谢相关mRNA表达进行测定。结果显示：相比低脂饮食，高脂饮食下小鼠的FBG、终体质量和体脂率显著增加，葡萄糖耐量和胰岛素耐量受损，血脂水平升高，肝细胞受损，肝脏TC、TG水平和血清ALT、AST活性总体无明显变化；高脂饮食下，与油茶籽油组相比，猪油组小鼠的胰岛素敏感性升高，肝细胞受损程度以及肝脏TG水平均降低，且猪油组的抑制肝脏脂质合成基因（Ampk）的mRNA表达上调，促进肝脏脂质合成基因（Acc1、Gpat4、Fasn、Scd1、Acc2）的mRNA表达下调，花生油组促进肝脏脂肪酸氧化（Pparα、Hsl、Atgl、Cpt1、Fatp4）和胆固醇代谢基因（Cyp7a1）mRNA显著或极显著上调，猪油组的糖异生基因（Foxo1、Cs和Pck1）的mRNA表达较花生油组极显著下调。综上，中国居民目前的油脂摄入水平会引起糖脂代谢紊乱，应适当减少油脂摄入，猪油对于糖脂代谢的作用效果优于花生油和油茶籽油。 |
| 英文摘要: |
| The aim is to examine the impact of the commonly used edible oils and the actual dietary fat energy level of the population on the glucose and lipid metabolism in human body to provide a reference basis for the selection of fats and oils for Chinese residents. Fifty-four C57BL/6J mice were averagely divided into six groups: three low-fat groups(15% fat energy diet, control diet) and three high-fat groups(35% fat energy diet, mimicking the actual dietary fat energy level of the population) receiving diets with lard, camellia seed oil and peanut oil as the added fat. All mice were maintained on their respective diets for 24 weeks, and the body weight of mice was recorded weekly. The glucose tolerance and insulin tolerance were measured at 22 and 23 weeks of feeding. At the end of the animal experiment, the fasting blood glucose (FBG) level, body fat percentage, and serum lipid level (TC, TG, LDL-C, HDL-C), liver pathology, liver TC and TG levels, serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities, and glucose and lipid metabolism-related mRNA levels were measured. The results showed that compared with a low-fat diet, a high-fat diet significantly increased FBG, final body weight, and body fat percentage, impaired glucose tolerance and insulin tolerance, elevated blood lipid levels, and damaged hepatocyte. However, there were no significant changes in liver TC and TG levels, and serum ALT and AST activities. At high-fat diet, compared with the camellia seed oil group, mice in the lard group exhibited improved insulin sensitivity, reduced hepatocyte damage and decreased liver TG level. Additionally, the lard group showed upregulated mRNA expression of the lipid synthesis-inhibiting gene Ampk and downregulated mRNA expression of lipid synthesis-promoting genes Acc1, Gpat4, Fasn, Scd1, and Acc2. The peanut oil group displayed significantly or highly significantly upregulated mRNA expression of fatty acid oxidation- and cholesterol metabolism-promoting genes Pparα, Hsl, Atgl, Cpt1, Fatp4 and Cyp7a1. The mRNA expression of gluconeogenic genes (Foxo1, Cs and Pck1) in the lard group was highly significantly downregulated compared with that in the peanut oil group. In conclusion, the current level of fat intake among Chinese residents can cause disorders in glucose and lipid metabolism. Therefore, fat intake should be appropriately reduced. Lard has a better effect on glucose and lipid metabolism than peanut oil and camellia seed oil. |
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