| 秘英静，岳珂，毕艳兰，张林尚，孙尚德，陈小威，李军.酯交换油脂的制备及其在发酵法生产UPU型
甘油三酯中的应用[J].中国油脂,2026,51(1):.[BI Yingjing, YUE Ke, BI Yanlan, ZHANG Linshang,
SUN Shangde, CHEN Xiaowei, LI Jun.Preparation of interesterified oil and their application in production of UPU-type triglycerides by fermentation method[J].China Oils and Fats,2026,51(1):.] |
| 酯交换油脂的制备及其在发酵法生产UPU型
甘油三酯中的应用 |
| Preparation of interesterified oil and their application in production of UPU-type triglycerides by fermentation method |
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| DOI：10.19902/j.cnki.zgyz.1003-7969.240718 |
| 中文关键词: UPU型甘油三酯 化学酯交换 浑浊红球菌 碳源调控 |
| 英文关键词:UPU-type triglycerides chemical interesterification Rhodococcus opacus carbon source regulation |
| 基金项目:河南省自然科学基金面上项目（242300421315）; 河南工业大学自然基金培育计划 （2024PYJH001） |
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| 摘要点击次数: 1901 |
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| 中文摘要: |
| 碳源对微生物的生长至关重要，旨在为发酵法生产UPU型甘油三酯的碳源选择提供参考，以富含棕榈酸的棕榈硬脂与富含亚油酸的红花籽油（质量比1∶ 2.47）为原料，通过化学酯交换得到酯交换油脂，以二者复配油脂为对照，考察了酯交换油脂的理化性质、脂肪酸组成和甘油酯组成，并研究以其为碳源时，浑浊红球菌的生长及产油特性。结果表明：与复配油脂相比，酯交换油脂色泽略微加深，滑动熔点、固体脂肪含量降低，sn-2位棕榈酸含量显著升高，sn-2位亚油酸含量显著降低，二者分别为32.39%和49.53%，甘油酯组成变化不大，三饱和型和三不饱和型甘油三酯含量显著降低；复配油脂相容性较差，棕榈硬脂易析出，浑浊红球菌难以吸收利用，生物量、含油率偏低；以复配油脂为碳源时，浑浊红球菌发酵所产油脂sn-2位棕榈酸含量仅为22.07%；以酯交换油脂为碳源时，浑浊红球菌生物量为2.03 g/L，含油率为41.71%，其发酵所产油脂sn-2位棕榈酸含量可达6054%，C52甘油三酯含量可达44.15%。综上，酯交换油脂在发酵法生产UPU型甘油三酯中具有优越性。 |
| 英文摘要: |
| Carbon source is crucial for fermentation. In order to provide guidance for selecting carbon sources for the fermentation-based production of UPU-type triglycerides, using palm stearin rich in palmitic acid and safflower seed oil rich in linoleic acid (mass ratio 1∶ 2.47) as raw materials, interesterified oil was obtained through chemical interesterification. A blended oil of palm stearin and safflower seed oil was used as a control, and the physicochemical properties, fatty acid composition, and glyceride composition of the interesterified oil were investigated. Additionally, the growth and oil production characteristics of Rhodococcus opacus were studied when using these oils as carbon sources. The results showed that compared to blended oil, interesterified oil exhibited a slightly darker color, lower slip melting point and solid fat content, a significant increase in sn-2 palmitic acid content (3239%) and a significant decrease in sn-2 linoleic acid content (49.53%), while the glyceride composition remained largely unchanged, with significant reductions in the contents of tri-saturated and tri-unsaturated triglycerides. Blended oil exhibited poor compatibility, with palm stearin tending to precipitate, making it difficult for R. opacus to absorb and utilize, resulting in lower biomass and oil content. When the blended oil was used as a carbon source, the sn-2 palmitic acid content of the bacterial oil was 22.07%. When the interesterified oil was used as a carbon source, the biomass, oil content, sn-2 palmitic acid content and total C52 content were 2.03 g/L, 41.71%, 60.54% and 4415%, respectively. In conclusion, the interesterified oil has superiority in the production of UPU-type triglycerides by fermentation method. |
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