| 方玉美1,2，赫玲玲1,2，程顺利1,2，张俊龙1,2，黄玉喜1,2，任秋鹤1,2， 赵绘婷1,2，肖进彬1,2.圆红冬孢酵母的高密度发酵产油过程研究[J].中国油脂,2026,51(1):.[FANG Yumei1,2, HE Lingling1,2, CHENG Shunli1,2, ZHANG Junlong1,2,
HUANG Yuxi1,2, REN Qiuhe1,2, ZHAO Huiting1,2, XIAO Jinbin1,2.High density fermentation process for oil production of Rhodosporidium toruloides[J].China Oils and Fats,2026,51(1):.] |
| 圆红冬孢酵母的高密度发酵产油过程研究 |
| High density fermentation process for oil production of Rhodosporidium toruloides |
| |
| DOI：10.19902/j.cnki.zgyz.1003-7969.240500 |
| 中文关键词: 微生物油脂 圆红冬孢酵母 产油 高密度发酵 脂肪酸分析 油脂产量 |
| 英文关键词:microbial oil Rhodosporidium toruloides oil production high density fermentation fatty acid analysis oil yield |
| 基金项目:河南省科学院基本科研费项目（230614050） |
| 作者 | 单位 | | 方玉美1,2，赫玲玲1,2，程顺利1,2，张俊龙1,2，黄玉喜1,2，任秋鹤1,2， 赵绘婷1,2，肖进彬1,2 | （1.河南省科学院,郑州 450002； 2.河南省高新技术实业有限公司,郑州 450002） |
|
| Author Name | Affiliation | | FANG Yumei1,2, HE Lingling1,2, CHENG Shunli1,2, ZHANG Junlong1,2,
HUANG Yuxi1,2, REN Qiuhe1,2, ZHAO Huiting1,2, XIAO Jinbin1,2 | (1.Henan Academy of Sciences, Zhengzhou 450002, China
2.Henan Hi-tech Industrial Corporation,
Zhengzhou 450002, China) |
|
| 摘要点击次数: 1816 |
| 全文下载次数: 425 |
| 中文摘要: |
| 为了探究圆红冬孢酵母的高密度发酵产油规律，用于调控油脂发酵过程以获得较大的生物量和油脂产量，在装液量30 L的50 L液态发酵罐中，采用生长代谢与发酵代谢两阶段发酵调控方式发酵圆红冬孢酵母，即在发酵0~42 h阶段溶解氧含量维持在40%~60%，温度32 ℃，pH 6.0，在发酵42~66 h阶段溶解氧含量维持在20%~30%，温度28 ℃，pH 6.0。对圆红冬孢酵母高密度发酵过程中生物量，碳源、氮源的消耗，油脂产量、脂肪得率系数、油脂脂肪酸组成，细胞内脂滴的形成及不同发酵阶段中富集通路的变化进行了研究。结果表明：圆红冬孢酵母高密度发酵结束时获得最大生物量为27.5 g/L，葡萄糖残留量为0.13 g/L，氮单位消耗量为0.524 g/L，油脂产量达11.935 g/L，其油脂中不饱和脂肪酸的含量高达61.656%；经葡萄糖消耗量和油脂产量计算得出，脂肪得率系数达到0.17 g/g；经激光共聚焦显微镜观察，发酵48 h时，脂质逐渐形成脂滴；由非靶向代谢分析得知，在发酵液中富集到的代谢通路中，精氨酸和脯氨酸代谢通路显著性影响最强，而在细胞内富集到的代谢通路中，ABC转运蛋白通路显著性影响最强。综上，通过两阶段（生长代谢与发酵代谢）溶解氧、温度调控策略，揭示了圆红冬孢酵母的高密度发酵产油过程中碳源、氮源的消耗规律，观察到脂滴的形成，并显著富集到与细胞生长及油脂合成相关代谢通路的变化。 |
| 英文摘要: |
| To explore the high density fermentation for oil production rules of Rhodosporidium toruloides and to regulate the lipid fermentation process to obtain a larger biomass and oil yield, Rhodosporidium toruloides was fermented in a 50 L liquid fermentation tank with a liquid capacity of 30 L, using a two-stage fermentation regulation method of growth metabolism and fermentation metabolism: the dissolved oxygen content was maintained at 40%-60% during the 0-42 h stage of fermentation, with a temperature of 32 ℃ and pH 6.0; during the 42-66 h stage of fermentation, the dissolved oxygen content was maintained at 20%-30%, with a temperature of 28 ℃ and pH 6.0. The biomass, carbon and nitrogen source consumption, oil yield, fat yield coefficient, fatty acid composition of oil, intracellular lipid droplet formation, and changes in enriched pathways at different fermentation stages during the high density fermentation of Rhodosporidium toruloides were studied. The results showed that at the end of the fermentation, the maximum biomass was 27.5 g/L, the residual glucose content was 0.13 g/L, the unit consumption of nitrogen was 0.524 g/L, and the oil yield reached 11.935 g/L, with unsaturated fatty acids accounting for as much as 61.656% of the oil content. Based on glucose consumption and oil yield calculations, the fat yield coefficient reached 0.17 g/g. Through confocal laser scanning microscope observation, lipid droplets gradually formed at 48 h of fermentation. Through non-targeted metabolic analysis, it was found that among the enriched metabolic pathways in the fermentation broth, the arginine and proline metabolic pathway had the strongest significant influence; among the enriched metabolic pathways in the cells, the ABC transporters pathway had the strongest significant influence. In conclusion, through the two-stage (growth metabolism and fermentation metabolism) dissolved oxygen and temperature regulation strategy, the consumption rules of carbon and nitrogen sources during the high density fermentation for oil production of Rhodosporidium toruloides are revealed, the formation of lipid droplets is observed, and the changes in metabolic pathways related to cell growth and lipid synthesis are significantly enriched. |
| 查看全文 查看/发表评论 下载PDF阅读器 |
| 关闭 |
|
|
|
