| 陈善辉1， 杨志荣1， 王东1， 黎仕友1， 刘仁彬1， 傅安静2， 李鹏婧1.富含OPL的结构脂对KM小鼠的润肠通便效果研究[J].中国油脂,2026,52(2):.[CHEN Shanhui1, YANG Zhirong1, WANG Dong1, LI Shiyou1,
LIU Renbin1, FU anjing2, LI Pengjing1.Laxative effect of structured lipids rich in OPL on KM mice[J].China Oils and Fats,2026,52(2):.] |
| 富含OPL的结构脂对KM小鼠的润肠通便效果研究 |
| Laxative effect of structured lipids rich in OPL on KM mice |
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投稿时间：2025-01-02 修订日期：2025-09-15 录用日期：2025-02-13
出版日期：2026-02-20
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| DOI：10.19902/j.cnki.zgyz.1003-7969.250001 |
| 中文关键词: OPL 结构脂 便秘 润肠通便 黏蛋白2 |
| 英文关键词:OPL structured lipids constipation laxative effect Mucin 2 |
| 基金项目:四川省成都市中试平台（合成生物产业领域）（2023-PT02-00061-CG） |
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| Author Name | Affiliation | | CHEN Shanhui1, YANG Zhirong1, WANG Dong1, LI Shiyou1,
LIU Renbin1, FU anjing2, LI Pengjing1 | 1.Synthetic Biology Engineering Center, Skuny BioScience Co. , Ltd. , Chengdu 611630, China；
2.Chengdu Agricultural College, Chengdu 611130, China |
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| 中文摘要: |
| 旨在为丰富结构脂的膳食应用提供理论依据，研究了富含1-油酸-2-棕榈酸-3-亚油酸甘油三酯（OPL）的结构脂（OPL/SL）对便秘模型KM小鼠的润肠通便效果及对结肠黏蛋白2（Muc2）表达的影响。采用盐酸洛哌丁胺建立小鼠便秘模型，设置空白对照组、阳性对照组、模型组和OPL/SL低（60 μL/10 g）、中（90 μL/10 g）、高（120 μL/10 g）剂量组（以OPL计），通过小鼠小肠运动实验和排便实验，观测OPL/SL对小鼠小肠墨汁推进率、排首粒黑便时间、24 h内排便数量及粪便含水率和小肠病理学的影响，同时测定了小肠组织的Muc2蛋白、结肠组织中Muc2基因及其表达。结果表明:在OPL/SL灌胃小鼠期间未造成小鼠生理指标的明显改变，体质量较为稳定；与模型组相比，各剂量组墨汁推进率有统计学差异（p<0.05，p<0.01），均能促进小肠运动；与模型组相比，各剂量组排首粒黑便时间均缩短，排便数量和粪便含水率均提高，有统计学意义（p<0.05，p<0.01），且中、高剂量组表现更好（p<0.01）；小鼠小肠病理学解剖结果显示，OPL/SL未造成肠道黏膜的不利影响；与模型组相比，各剂量组小肠绒毛高度与隐窝深度比值及小肠杯状细胞数量均提高，表明OPL/SL降低了盐酸洛哌丁胺的不利影响；Muc2及其蛋白表达水平结果表明，与模型组相比，各剂量组有统计学差异（p<0.05，p<0.01），均能提高Muc2及其蛋白表达，尤其是高剂量组表现更好，与其他剂量组相比显示出一定的量效关系。综上，OPL/SL具有良好的润肠通便作用。 |
| 英文摘要: |
| In order to provide a theoretical basis for expanding the dietary applications of structured lipids, the laxative effect of structured lipids rich in 1-oleoyl-2-palmitoyl-3-linoleoylglycerol (OPL/SL) on constipated KM mice and its influence on colonic mucin 2 (Muc2) expression were investigated. A constipation model was established using loperamide hydrochloride. Mice were divided into blank control, positive control, model, and OPL/SL treatment groups with low-(60 μL/10 g), medium-(90 μL/10 g), and high-(120 μL/10 g) doses(based on OPL). Through small intestine propulsion and defecation tests, the effects of OPL/SL on intestinal ink propulsion rate, time to first black stool defecation, number of fecal pellets within 24 h, fecal water content, and intestinal pathology were observed. Meanwhile, the expression of Muc2 protein in intestine tissue and Muc2 gene and its protein in colon tissue was detected. The results showed that during the OPL/SL administration in mice did not cause significant changes in physiological indicators, and body weight remained stable. Compared with the model group, all dose groups showed statistically significant differences in intestinal ink propulsion rate (p<0.05, p<0.01), indicating promoted intestinal motility. Compared with the model group, the time to first black stool defecation shortened, and both fecal count and water content increased with statistical significance (p<0.05, p<0.01) for the dose groups, while the medium-and high-dose groups performed even better (p<0.01). Pathological examination revealed no adverse effects of OPL/SL on the intestinal mucosa. Compared with the model group, all dose groups showed an increased ratio of villus height to crypt depth and a higher number of goblet cells, suggesting that OPL/SL reduced the harmful effects of loperamide hydrochloride. Results of Muc2 gene and its protein expression showed that all dose groups significantly up-regulated Muc2 and its protein expression compared with the model group (p<0.05, p<0.01), with the high-dose group showing the best effect and a certain dose-response relationship. In conclusion, OPL/SL has a significant laxative effect. |
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