| 邓婷婷1,柳远哲1,杨凌寒1,张东慧1,唐盛2,3,王强3，4,陈喜锋2,4,梁剑光1.一株来源于餐厨垃圾的高产脂肪酶菌株的
筛选、鉴定及其产酶条件优化[J].中国油脂,2026,51(4):.[DENG Tingting1, LIU Yuanzhe1, YANG Linghan1, ZHANG Donghui1,
TANG Sheng2,3, WANG Qiang3,4, CHEN Xifeng2,4, LIANG Jianguang1.Screening, identification, and enzyme production condition optimization of a high lipase-producing strain derived from kitchen waste[J].China Oils and Fats,2026,51(4):.] |
| 一株来源于餐厨垃圾的高产脂肪酶菌株的
筛选、鉴定及其产酶条件优化 |
| Screening, identification, and enzyme production condition optimization of a high lipase-producing strain derived from kitchen waste |
| |
| DOI：10.19902/j.cnki.zgyz.1003-7969.240701 |
| 中文关键词: 餐厨垃圾 脂肪酶 油脂降解 空气芽孢杆菌 产酶工艺优化 响应面法 |
| 英文关键词:kitchen waste lipase oil-degrading Bacillus aerius optimization of enzyme production process response surface methodology |
| 基金项目:江苏省研究生科研实践创新计划（KYCX21_2866） |
| 作者 | 单位 | | 邓婷婷1,柳远哲1,杨凌寒1,张东慧1,唐盛2,3,王强3，4,陈喜锋2,4,梁剑光1 | 1.常州大学 药学院 生物与食品工程学院,江苏 常州 213164； 2.常州源康生物科技有限公司,江苏 常州 213164；
3.苏州源康生物科技有限公司,江苏 苏州 215000； 4.江苏绿博生物科技有限公司,江苏 常熟 215500 |
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| Author Name | Affiliation | | DENG Tingting1, LIU Yuanzhe1, YANG Linghan1, ZHANG Donghui1,
TANG Sheng2,3, WANG Qiang3,4, CHEN Xifeng2,4, LIANG Jianguang1 | 1.School of Pharmacy & School of Biological and Food Engineering, Changzhou University, Changzhou
213164, Jiangsu, China
2.Changzhou Yuankang Biotech Co. , Ltd. , Changzhou 213164,
Jiangsu, China
3.Suzhou Yuankang Biotech Co. , Ltd. , Suzhou 215000, Jiangsu, China
4.Jiangsu Lvbo Biotech Co. , Ltd. , Changshu 215500, Jiangsu, China |
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| 中文摘要: |
| 旨在为富含油脂的餐厨垃圾的高效分解提供参考，从餐厨垃圾中筛选出高产脂肪酶的菌株，通过16S rDNA测序并构建发育树来鉴定菌株种属，并利用单因素实验和响应面实验优化产酶条件。结果表明：从餐厨垃圾中共分离出16株具有油脂降解能力的菌株，其中1株菌株的油脂降解率和脂肪酶酶活分别达到了76.61%和380.55 U/mL，将其命名为CZU-JY13；分子学鉴定结果显示，该菌株属于空气芽孢杆菌（Bacillus aerius）；菌株CZU-JY13的最优产酶条件为培养基初始pH 9.9、发酵温度37.0 ℃、培养基中NaCl质量浓度4.6 g/L、接种量3%、发酵时间48 h，在此条件下脂肪酶酶活高达（620.56±5.34） U/mL，较优化前提高63.07%。综上，菌株CZU-JY13是一种可以高效处理餐厨垃圾中油脂的菌种资源。 |
| 英文摘要: |
| To provide a reference for the efficient decomposition of oily kitchen waste, a high lipase-producing strain was screened from kitchen waste, identified via 16S rDNA sequencing and phylogenetic tree construction, and its enzyme production conditions were optimized using single-factor experiments and response surface methodology. The results showed that a total of 16 strains with oil-degrading ability were isolated from kitchen waste, among which one strain exhibited a lipid degradation rate of 76.61% and a lipase activity of 380.55 U/mL. This strain was designated CZU-JY13. Molecular identification revealed that strain CZU-JY13 belonged to Bacillus aerius. The optimal enzyme production conditions for strain CZU-JY13 were determined as follows: medium initial pH 9.9, fermentation temperature 37.0 ℃, NaCl mass concentration 4.6 g/L, inoculation volume 3%, and fermentation time 48 h. Under these optimized conditions, lipase activity reached (620.56 ± 534) U/mL, representing a 63.07% increase compared to pre-optimization levels. In conclusion, strain CZU-JY13 is a microbial resource with high potential for efficient degradation of oils in kitchen waste. |
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