| 杨勇1，何帮翔1,2，张琴1,2，吴晓龙1,2，李艳宾1,2.微藻基碳量子点制备及其调控微藻生长
与油脂积累研究[J].中国油脂,2026,51(4):.[YANG Yong1, HE Bangxiang1,2, ZHANG Qin1,2，WU Xiaolong1,2, LI Yanbin1,2.Preparation of microalgae-based carbon quantum dots and their regulatory effects on microalgal growth and lipid accumulation[J].China Oils and Fats,2026,51(4):.] |
| 微藻基碳量子点制备及其调控微藻生长
与油脂积累研究 |
| Preparation of microalgae-based carbon quantum dots and their regulatory effects on microalgal growth and lipid accumulation |
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| DOI：10.19902/j.cnki.zgyz.1003-7969.250096 |
| 中文关键词: 碳量子点 水热合成 微藻 生长 油脂 |
| 英文关键词:carbon quantum dots hydrothermal synthesis microalgae growth lipids |
| 基金项目:安徽省高校优秀青年人才支持计划重点项目（gxyqZD2022049）；安徽省自然科学基金项目（2108085MB37）；安徽省重点研究与开发计划项目（202004i07020003） |
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| Author Name | Affiliation | | YANG Yong1, HE Bangxiang1,2, ZHANG Qin1,2，WU Xiaolong1,2, LI Yanbin1,2 | 1.School of Biological and Food Engineering, Anhui Polytechnic University, Wuhu 241000,
Anhui, China； 2.Anhui Engineering Laboratory for Industrial Microbiology
Molecular Breeding, Wuhu 241000, Anhui, China |
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| 中文摘要: |
| 为实现微藻资源高值化利用，以提取油脂后的小球藻藻渣为碳源，通过水热合成法分别制备微藻基未掺杂碳量子点（M-CQDs）和硫掺杂碳量子点（S-CQDs），对其进行表征，并探讨两种碳量子点对小球藻Chlorella sp. DT025生长及产油的影响。结果表明：M-CQDs和S-CQDs平均粒径分别为2.31 nm和2.72 nm，均处于碳量子点典型粒径范围，且均呈单分散状态，但S-CQDs的荧光特性（发射波长）发生红移；与对照组相比，在5 000 lx光照强度下，添加10 mg/L的M-CQDs可使小球藻生物量提高1050%，油脂含量提高61.88%，达到289.74 mg/g；而添加10 mg/L的 S-CQDs对8 000 lx光照强度下的小球藻的生物量和油脂含量有较为显著的促进作用，相比对照组分别提高了553%和3309%；添加M-CQDs的小球藻脂肪酸中C16~C18脂肪酸占比最高，达到8537%，其中C18∶ 1含量相比对照组增加19.36%。综上，制备的微藻基碳量子点可应用于产油微藻培养过程的调控与改善。 |
| 英文摘要: |
| To achieve high-value utilization of microalgal resources, microalgae-based undoped carbon quantum dots (M-CQDs) and sulfur-doped carbon quantum dots (S-CQDs) were synthesized via a hydrothermal method using lipid-extracted Chlorella residue as the carbon source. Their properties were characterized, and the effects of M-CQDs and S-CQDs on the growth and lipid accumulation of Chlorella sp. DT025 were systematically investigated. The results showed that both M-CQDs and S-CQDs exhibited typical carbon quantum dot partical size, measuring 2.31 nm and 2.72 nm, respectively, with monodisperse distribution. Compared to M-CQDs, S-CQDs demonstrated a red shift in fluorescence characteristics(emission wavelength). Under 5 000 lx illumination, the addition of 10 mg/L M-CQDs enhanced the biomass of Chlorella by 10.50% and increased lipid content by 6188% compared to the control group, reaching 289.74 mg/g. Meanwhile, the addition of 10 mg/L S-CQDs significantly promoted the biomass and lipid content of Chlorella under 8 000 lx illumination, with an increase of 5.53% and 33.09% respectively compared to the control group.Fatty acid composition analysis revealed that the M-CQDs-treated group exhibited the highest proportion of C16-C18 fatty acids (8537%), with C18∶ 1 content increasing by 19.36% relative to the control group. In conclusion, the prepared microalgae-based carbon quantum dots can be applied to regulate and improve the cultivation process of oil producing microalgae. |
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