| 王法1， 李嘉怡1， 幸勇2， 张清1.豌豆分离蛋白-葡聚糖-原花青素复合物
结构与功能研究[J].中国油脂,2026,51(4):.[WANG Fa1, LI Jiayi1, XING Yong2, ZHANG Qing1.Structural and function of ternary complex of pea protein isolate-dextran-proanthocyanidins[J].China Oils and Fats,2026,51(4):.] |
| 豌豆分离蛋白-葡聚糖-原花青素复合物
结构与功能研究 |
| Structural and function of ternary complex of pea protein isolate-dextran-proanthocyanidins |
| |
| DOI：10.19902/j.cnki.zgyz.1003-7969.250035 |
| 中文关键词: 豌豆分离蛋白-葡聚糖轭合物 原花青素 三元复合物 结构 功能性质 |
| 英文关键词:pea protein isolate-dextran conjugates proanthocyanidins ternary complex structure functional property |
| 基金项目:2023年度四川省留学归国人员科技活动项目择优资助项目（2322999038） |
|
| Author Name | Affiliation | | WANG Fa1, LI Jiayi1, XING Yong2, ZHANG Qing1 | 1.College of Food Science, Sichuan Agricultural University, Ya′an 625000, Sichuan,China
2.Sichuan Ng Fung Li Hong Food Co. , Ltd. , Ya′an 625300, Sichuan, China |
|
| 摘要点击次数: 412 |
| 全文下载次数: 204 |
| 中文摘要: |
| 为开发新型生物活性成分递送系统，制备豌豆分离蛋白-葡聚糖轭合物（PDC），并分别与原花青素经共价、非共价结合得到三元复合物，研究豌豆分离蛋白-葡聚糖-原花青素复合物的结构及功能性质。结果表明：与PDC相比，豌豆分离蛋白-葡聚糖-原花青素复合物的二、三级结构发生了变化，三元复合物均呈现球形纳米颗粒状，且原花青素添加量为0.10%时（以PDC溶液质量计）共价三元复合物具有更高的多酚结合当量（113.2 μmol/g）（以PDC质量计）；同一原花青素添加量下共价三元复合物的抗氧化活性强于非共价三元复合物的,其DPPH和ABTS+自由基清除率最大分别为89.00%和90.32%，而非共价三元复合物的分别为86.12%、89.38%；模拟胃肠道消化实验表明，共价三元复合物中的原花青素在胃内能得到有效保护，在肠道可充分释放，消化150 min时多酚释放量达142.9 mg/g。综上，豌豆分离蛋白-葡聚糖-原花青素共价三元复合物作为递送载体对生物活性物质具有良好的保护作用，可作为新型递送系统开发利用。 |
| 英文摘要: |
| In order to develop a new type of bioactive component delivery system, pea protein isolate-dextran conjugates (PDC) were prepared, and they were covalently and non-covalently combined with proanthocyanidins respectively to obtain ternary complexes. The structure and functional properties of the pea protein isolate-dextran-proanthocyanidins complex were studied. The results showed that compared with PDC, the secondary and tertiary structures of the pea protein isolate-dextran-proanthocyanidins complex changed. The ternary complex all presented spherical nanoparticles, and when the the proanthocyanidin dosage was 0.10%( based on the mass of PDC solution), covalent ternary complex had a higher polyphenol bound equivalent (113.2 μmol/g). Under the same dosage of proanthocyanidins, the antioxidant activity of the covalent ternary complex was stronger than that of the non-covalent ternary complex. The maximum DPPH and ABTS+ free radical scavenging rates of covalent ternary complex were 89.00% and 90.32% respectively, while those of the non-covalent ternary complex were 8612% and 89.38% respectively. Simulated gastrointestinal digestion experiments indicated that proanthocyanidins in the covalent ternary complex could be effectively protected in the stomach and fully released in the intestine. At 150 min of digestion, the polyphenol release amount reached 142.9 mg/g. In conclusion, pea protein isolate-dextran-proanthocyanidins covalent ternary complex, as a delivery carrier, has a good protective effect on bioactive substances and can be developed and utilized as a new delivery system. |
| 查看全文 查看/发表评论 下载PDF阅读器 |
| 关闭 |
|
|
|
