| 刘普芳，包音都古荣·金花，郭军，秦嘉杉，闫鑫磊.苏尼特羊尾油脂对小鼠溃疡性结肠炎的缓解作用研究[J].中国油脂,2026,51(5):.[LIU Pufang, BAOYINDUGURONG Jinhua, GUO Jun,
QIN Jiashan, YAN Xinlei.Alleviating effect of Sunite sheep tail oil on mice with ulcerative colitis[J].China Oils and Fats,2026,51(5):.] |
| 苏尼特羊尾油脂对小鼠溃疡性结肠炎的缓解作用研究 |
| Alleviating effect of Sunite sheep tail oil on mice with ulcerative colitis |
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| DOI：10.19902/j.cnki.zgyz.1003-7969.250116 |
| 中文关键词: 苏尼特羊尾油脂 溃疡性结肠炎 炎症因子 抗氧化 肠道屏障功能 肠道菌群 |
| 英文关键词:Sunite sheep tail oil ulcerative colitis inflammatory cytokines antioxidant intestinal barrier function gut microbiota |
| 基金项目:内蒙古自治区科技计划项目（2021GG0348） |
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| 中文摘要: |
| 旨在为膳食干预缓解肠道炎症提供参考，探讨了苏尼特羊尾油脂（STO）对小鼠溃疡性结肠炎（UC）的缓解作用。将BALB/c雄性小鼠随机均分为空白（NC）组、模型（D）组、STO-D组、苏尼特羊肾周脂肪（SPF-D）组、猪油（LO-D）组和菜籽油（RO-D）组，前2组饲喂普通饲料，后4组分别饲喂STO、苏尼特羊肾周脂肪、猪油、菜籽油添加量为10%的普通饲料，实验周期21 d，1~14 d各组小鼠自由饮水，15~21 d NC组小鼠自由饮水，其余组小鼠饮用3.5%葡聚糖硫酸钠盐溶液，以建立UC小鼠模型。通过对小鼠基本生长情况分析、结肠组织病理学观察、血清炎症因子水平和结肠氧化应激指标检测、免疫组化检测和肠道菌群分析，探讨STO对小鼠UC的缓解作用。结果表明：STO可缓解UC小鼠结肠缩短和体质量减轻情况，显著降低UC小鼠的疾病活动度指数；STO可改善UC小鼠的肠道损伤与炎性细胞浸润现象，促进肠道组织恢复；STO干预使UC小鼠血清肿瘤坏死因子-α、白细胞介素-1β和结肠丙二醛（MDA）水平显著下降（p＜0.05），结肠超氧化物歧化酶（SOD）活性显著上升（p＜0.05）；STO干预促使小鼠结肠细胞连接蛋白claudin-1、occludin、E-cadherin和ZO-1的表达水平显著增加（p＜0.05）；同时，STO可改善UC小鼠肠道菌群结构，丰富潜在有益菌属如拟杆菌属、副拟杆菌属等，恢复厚壁菌门与拟杆菌门比值。综上，STO可通过减少炎症因子、增强体内抗氧化水平、调节肠道屏障功能及丰富肠道菌群构成有效改善小鼠UC。 |
| 英文摘要: |
| In order to provide a reference for dietary intervention in intestinal inflammation, the alleviating effect of Sunite sheep tail oil (STO) on mice with ulcerative colitis (UC) was investigated. Male BALB/c mice were randomly and evenly divided into blank (NC) group, model (D) group, STO-D group, Sunite perirenal fat(SPF-D) group, lard (LO-D) group and rapeseed oil (RO-D) group. The first two groups were fed with normal diet, while the latter four groups were respectively fed with normal diet with 10% STO, Sunite perirenal fat, lard and rapeseed oil addition. The experiment lasted for 21 d. From 1-14 d, all mice had free access to water. From 15-21 d, the NC group continued with free drinking water, while the other groups were given 3.5% dextran sulfate sodium (DSS) solution to induce UC. The alleviating effect of STO on mice with UC was evaluated by analyzing basic growth conditions, colon histopathology, serum inflammatory cytokine levels, colonic oxidative stress indicators, immunohistochemistry, and gut microbiota composition. The results showed that STO alleviated colon shortening and body weight loss in UC mice and significantly reduced the disease activity index. STO also improved intestinal damage and inflammatory infiltration, promoting the restoration of colon tissue. STO intervention significantly decreased serum levels of TNF-α and IL-1β and colonic malondialdehyde (MDA) content (p<0.05), while increased colonic superoxide dismutase (SOD) activity (p<0.05). Furthermore, STO significantly upregulated the expression of cell junction proteins in the colon, including claudin-1, occludin, E-cadherin, and ZO-1 (p<0.05). Additionally, STO modulated the gut microbiota structure in UC mice by enriching potential beneficial bacteria such as Bacteroides and Parabacteroides, and restoring the Firmicutes/Bacteroidota ratio. In conclusion, the alleviating effect of STO on UC mice can be achieved by reducing inflammatory cytokines, enhancing the in vivo antioxidant capacity, regulating the intestinal barrier function, and optimizing the composition of the gut microbiota. |
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