| 董杨帅1，徐思渊1，于博安1，李艳艳1，陈小红2，李红娟1，于景华1，李洪波1.高压均质处理下β-乳球蛋白/亚油酸复合物的
结构变化及其制备工艺优化[J].中国油脂,2026,51(5):.[DONG Yangshuai1, XU Siyuan1, YU Bo′an1, LI Yanyan1, CHEN Xiaohong2,
LI Hongjuan1, YU Jinghua1, LI Hongbo1.Structural changes and process optimization of β-lactoglobulin/linoleic acid complexes under high-pressure homogenization[J].China Oils and Fats,2026,51(5):.] |
| 高压均质处理下β-乳球蛋白/亚油酸复合物的
结构变化及其制备工艺优化 |
| Structural changes and process optimization of β-lactoglobulin/linoleic acid complexes under high-pressure homogenization |
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| DOI：10.19902/j.cnki.zgyz.1003-7969.250147 |
| 中文关键词: β-乳球蛋白 亚油酸 高压均质 结构特性 |
| 英文关键词:β-lactoglobulin linoleic acid high-pressure homogenization structural characteristics |
| 基金项目:天津市科技计划项目（23JCYBJC01160，23YDTTPJC00340） |
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| Author Name | Affiliation | | DONG Yangshuai1, XU Siyuan1, YU Bo′an1, LI Yanyan1, CHEN Xiaohong2,
LI Hongjuan1, YU Jinghua1, LI Hongbo1 | 1.School of Food Science and Engineering, Tianjin University of Science and Technology,
Tianjin 300457, China
2.Tianjin Haihe Dairy Co. , Ltd. , Tianjin 300457, China |
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| 中文摘要: |
| 旨在为开拓和研发更具营养价值的食品提供参考,以不同均质压力条件下制备的β-乳球蛋白（β-LG）/亚油酸复合物为研究对象，利用光谱学方法探究均质压力对复合物结构特性的影响，并在预实验的基础上,以底物（β-LG与亚油酸）物质的量比、反应时间、均质压力为因素，以β-LG/亚油酸复合物的粒径为响应值，通过响应面法优化β-LG/亚油酸复合物的制备工艺。结果表明：随着均质压力（0～120 MPa)的增加，β-LG/亚油酸复合物的内源荧光强度先降后升，紫外吸收和表面疏水性先升后降，α-螺旋含量先下降后上升，β-折叠含量先升高后下降；β-LG/亚油酸复合物制备的最优工艺条件为反应时间10 min、均质压力80 MPa、β-LG与亚油酸物质的量比1∶ 1，在此条件下β-LG/亚油酸复合物的粒径为210.26 nm。综上，β-LG/亚油酸复合物的结构特性会因高压均质处理而发生显著改变。 |
| 英文摘要: |
| To provide a reference for the development of nutritionally enhanced foods, with β-lactoglobulin (β-LG)/linoleic acid complexes prepared under different homogenization pressures as the material, the effect of homogenization pressure on the structural characteristics of β-LG/linoleic acid complexes were studied using spectroscopic techniques. Based on preliminary experiments, response surface methodology was employed to optimize the preparation conditions of the complexes, with the molar ratio of β-LG to linoleic acid, reaction time, and homogenization pressure as independent variables, and the particle size of the complexes as the response value. The results showed that as the homogenization pressure increased from 0 MPa to 120 MPa, the intrinsic fluorescence intensity of the complexes first decreased and then increased, while the UV absorption and surface hydrophobicity first increased and then decreased. Similarly, the α-helix content initially decreased and then increased, whereas the β-sheet content showed the opposite trend. The optimal preparation conditions were determined to be a reaction time of 10 min, homogenization pressure of 80 MPa, and a β-LG to linoleic acid molar ratio of 1∶ 1, yielding a complex particle size of 210.26 nm. In conclusion, high-pressure homogenization significantly alters the structure of β-LG/linoleic acid complexes. |
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