| 骆征山1,2,3，唐顺之1,2,3，彭万才1,2,3，文舒琪1,2,3，招振楠1,2,3，
牟肖男1,2,3，甘勇4，许文东1,2,3.磷脂酶D催化合成二硬脂酰磷脂酰乙醇胺[J].中国油脂,2026,51(5):.[LUO Zhengshan1,2,3，TANG Shunzhi1,2,3，PENG Wancai1,2,3，WEN Shuqi1,2,3，
ZHAO Zhennan1,2,3，MU Xiaonan1,2,3，GAN Yong4，XU Wendong1,2,3.Catalytic synthesis of distearoylphosphatidylethanolamine by phospholipase D[J].China Oils and Fats,2026,51(5):.] |
| 磷脂酶D催化合成二硬脂酰磷脂酰乙醇胺 |
| Catalytic synthesis of distearoylphosphatidylethanolamine by phospholipase D |
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| DOI：10.19902/j.cnki.zgyz.1003-7969.250025 |
| 中文关键词: 磷脂酶D 酶催化 二硬脂酰磷脂酰乙醇胺 合成磷脂 |
| 英文关键词:phospholipase D enzyme catalysis distearoylphosphatidylethanolamine synthetic phospholipid |
| 基金项目:广东省科技计划项目“广东省药用脂质重点实验室”（2023B1212070030） |
| 作者 | 单位 | | 骆征山1,2,3，唐顺之1,2,3，彭万才1,2,3，文舒琪1,2,3，招振楠1,2,3，
牟肖男1,2,3，甘勇4，许文东1,2,3 | 1.广州白云山汉方现代药业股份有限公司,广州 510240； 2.中药制药过程技术与新药创制国家工程研究中心,广州 510240；
3.广东省药用脂质重点实验室,广州 510240； 4.中国科学院上海药物研究所,原创新药研究全国重点实验室,上海 201210 |
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| Author Name | Affiliation | | LUO Zhengshan1,2,3，TANG Shunzhi1,2,3，PENG Wancai1,2,3，WEN Shuqi1,2,3，
ZHAO Zhennan1,2,3，MU Xiaonan1,2,3，GAN Yong4，XU Wendong1,2,3 | 1.Guangzhou Baiyunshan Hanfang Pharmaceutical Co. , Ltd. , Guangzhou 510240, China
2.National Engineering
Research Center of Pharmaceutical Processing Technology of Traditional Chinese Medicine and Drug Innovation,
Guangzhou 510240, China
3.Guangdong Provincial Key Laboratory of Medicinal Lipid, Guangzhou 510240,
China
4.National Key Laboratory of Original Innovative Drug Research, Shanghai Institute of
Materia Medica, Chinese Academy of Sciences, Shanghai 201210, China |
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| 中文摘要: |
| 为了探究磷脂酶D（PLD）催化合成二硬脂酰磷脂酰乙醇胺（DSPE）的可行性，采用PLD催化二硬脂酰磷脂酰胆碱（DSPC）合成DSPE，并通过乙酸乙酯洗涤和柱层析法进行纯化，通过单因素实验对工艺条件（有机溶剂种类、pH、反应温度、有机相与水相体积比、酶用量、底物物质的量比、反应时间）进行优化。结果表明：酶法合成DSPE的最佳反应条件为以二氯甲烷为有机溶剂、pH 5.5、反应温度30 ℃、有机相与水相体积比1∶ 1、酶用量60%（以DSPC质量计）、底物乙醇胺与DSPC物质的量比40∶ 1、反应时间12 h，在此条件下DSPE的产率为92.1%；DSPE成品的纯度为9922%。综上，该工艺条件温和，产率高，可用于DSPE的制备。 |
| 英文摘要: |
| In order to explore the feasibility of phospholipase D (PLD) catalyzed synthesis of distearoylphosphatidylethanolamine (DSPE), the DSPE was synthesized from distearoylphosphatidylcholine (DSPC) catalyzed by PLD, and the resulting product was purified via ethyl acetate washing followed by column chromatography. The process conditions (organic solvent, pH, reaction temperature, volume ratio of organic phase to aqueous phase, enzyme dosage, substrate molar ratio, and reaction time) were optimized through a single-factor experiment. The results showed that the optimum reaction conditions for enzymatic synthesis of DSPE were as follows: using dichloromethane as the organic solvent, pH 5.5, reaction temperature 30 ℃, volume ratio of organic phase to aqueous phase 1∶ 1, enzyme dosage 60% (based on the mass of DSPC), molar ratio of ethanolamine to DSPC 40∶ 1, and reaction time 12 h. Under the optimum reaction conditions, the yield of DSPE was 92.1% and the purity of the finished product DSPE was 99.22%. In summary, this process offers mild conditions and high yields, making it suitable for the preparation of DSPE. |
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