| 张良晨，田圆，于淼.大豆油甘油二酯油的酶法甘油解制备
与亚临界萃取纯化工艺研究[J].中国油脂,2026,51(8):.[ZHANG Liangchen, TIAN Yuan, YU Miao.Enzymatic glycerolysis preparation and subcritical extraction purification process of soybean oil-based diglycerides oil[J].China Oils and Fats,2026,51(8):.] |
| 大豆油甘油二酯油的酶法甘油解制备
与亚临界萃取纯化工艺研究 |
| Enzymatic glycerolysis preparation and subcritical extraction purification process of soybean oil-based diglycerides oil |
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投稿时间：2025-06-27 修订日期：2026-04-28 录用日期：2025-08-22
出版日期：
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| DOI：10.19902/j.cnki.zgyz.1003-7969.250302 |
| 中文关键词: 大豆油 甘油二酯 亚临界萃取 响应面法 |
| 英文关键词:soybean oil diglycerides subcritical extraction response surface methodology |
| 基金项目:辽宁省重点研发计划项目（2024JH2/102400004）；辽宁省农业科学院学科建设项目（2025XKJS8533） |
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| 中文摘要: |
| 旨在为大豆油甘油二酯（DAG）油的工业化生产及应用提供理论依据和技术指导，采用酶法甘油解制备大豆油DAG油，采用亚临界萃取技术对大豆油DAG油进行纯化，采用单因素试验和响应面试验优化制备和纯化工艺条件，并与原料大豆油的甘油酯、脂肪酸组成及理化指标进行对比。结果表明：大豆油DAG油的最佳制备工艺条件为大豆油与甘油、水质量比1∶ 0.5∶ 0.3，酶添加量080%（以大豆油质量计），反应温度56 ℃，反应时间8 h，在此条件下制备的大豆油DAG油中DAG含量为31.65%；大豆油DAG油的最佳纯化工艺为大豆油DAG油与丁烷质量比1∶ 1.5、萃取温度59 ℃、萃取时间55 min，纯化后DAG油中DAG含量达60.23%；大豆油DAG油主要由甘油三酯和DAG组成，未检出甘油一酯；其脂肪酸组成与大豆油类似；大豆油DAG油的酸值、过氧化值和碘值与原料大豆油没有显著差异。综上，将亚临界萃取技术应用于大豆油DAG油的纯化可以有效提高其DAG含量，且未改变油脂本身的理化指标。 |
| 英文摘要: |
| To provide a theoretical basis and technical guidance for the industrial production and application of soybean oil-based diglycerides (DAG) oil, the DAG oil was prepared from soybean oil using an enzymatic glycerolysis process and purified using subcritical extraction technology. Based on single-factor experiments, response surface experiments were conducted to optimize the preparation and purification process conditions, and the glyceride and fatty acid compositions as well as the physicochemical properties of DAG oil were compared with those of the raw soybean oil. The results showed that the optimal preparation conditions for DAG oil were soybean oil-glycerol-water mass ratio 1∶ 0.5∶ 0.3, enzyme dosage 0.80%(based on the mass of soybean oil), reaction temperature 56 ℃, and reaction time 8 h. Under these conditions, the DAG content in the product was 31.65%. The optimal purification conditions for DAG oil were DAG oil-to-butane mass ratio 1∶ 1.5, extraction temperature 59 ℃, and extraction time 55 min, resulting in a DAG content of 60.23% after purification. The prepared DAG oil consisted of TAG and DAG, with no MAG detected, and its fatty acid composition was similar to that of soybean oil. The acid value, peroxide value, and iodine value of the DAG oil showed no significant differences from those of the raw soybean oil. In summary, the application of subcritical extraction technology to the purification of soybean oil-based DAG oil can effectively increase its DAG content without altering the physicochemical properties of the oil itself. |
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