| 张小龙，何利，代寻，刘馨，李建龙，胡凯弟，刘书亮，陈姝娟.磁性固定化脂肪酶Pickering乳液的制备
及其在催化大豆油水解中的应用[J].中国油脂,2026,51(8):.[ZHANG Xiaolong，HE Li，DAI Xun，LIU Xin，LI Jianlong，
HU Kaidi，LIU Shuliang，CHEN Shujuan.Preparation of magnetic immobilized lipase Pickering emulsion and its application in catalyzing soybean oil hydrolysis[J].China Oils and Fats,2026,51(8):.] |
| 磁性固定化脂肪酶Pickering乳液的制备
及其在催化大豆油水解中的应用 |
| Preparation of magnetic immobilized lipase Pickering emulsion and its application in catalyzing soybean oil hydrolysis |
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投稿时间：2025-11-25 修订日期：2026-04-11 录用日期：2026-01-14
出版日期：
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| DOI：10.19902/j.cnki.zgyz.1003-7969.250503 |
| 中文关键词: 固定化脂肪酶 Pickering乳液 脂肪酸制备 Fe3O4纳米颗粒 |
| 英文关键词:immobilized lipase Pickering emulsion preparation of fatty acids Fe3O4 nanoparticles |
| 基金项目:四川省产教融合示范项目“饲料工业全产业链转型升级产教融合创新示范”（000-2212129406） |
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| 中文摘要: |
| 为提高固定化脂肪酶在两相催化体系中的催化效率，以硅烷修饰的磁性Fe3O4纳米颗粒为载体，分别采用吸附交联和共价结合两种方法制备磁性固定化脂肪酶，采用傅里叶变换红外光谱（FTIR）仪、差示扫描量热（DSC）仪、X射线衍射（XRD）仪和扫描电子显微镜（SEM）对其进行表征，并测定其粒径与Zeta电位。在此基础上，分别制备吸附交联法固定化脂肪酶（C@FS-AC）Pickering乳液和共价结合法固定化脂肪酶（C@FS-CB）Pickering乳液，并以其为催化剂催化大豆油水解，评价其催化效率，并考察了2种Pickering乳液催化体系中固定化脂肪酶的重复利用性、贮藏稳定性、催化油脂水解反应的稳定性及金属离子和有机分子对其催化油脂水解反应的影响。结果表明：通过表征证明脂肪酶成功负载于Fe3O4上；与游离脂肪酶和固定化脂肪酶相比，C@FS-AC Pickering乳液和C@FS-CB Pickering乳液催化体系在1 h内催化大豆油水解效率分别提高了2.7倍与8.6倍、4.1倍与46.1倍；C@FS-AC Pickering乳液和C@FS-CB Pickering乳液催化体系在15 d内均有良好的稳定性，在循环利用6次后其中的固定化脂肪酶的催化活性仍能分别保持78.79%和80.40%；2种Pickering乳液催化油脂水解效率因油脂饱和度差异而不同，且其催化体系的最佳温度为35 ℃，在前25 h具有较快催化效率；低含量的Fe3+和没食子酸对Pickering乳液催化大豆油水解具有一定促进作用，含量过高时则有抑制作用，而Cu2+和姜黄素则具有抑制作用，维生素E对其无明显影响。综上，磁性固定化脂肪酶Pickering乳液催化体系具有较好的稳定性和抗干扰能力，在油水两相催化中具有很好的催化效率。 |
| 英文摘要: |
| To improve the catalytic efficiency of immobilized lipase in a two-phase catalytic system, silylated magnetic Fe3O4 nanoparticles as a carrier to prepare two kinds of magnetic immobilized lipase by adsorption-crosslinking and covalent bonding, they were systematically characterized by FTIR, DSC, XRD, SEM, and their particle size distribution and Zeta potential were determined. Pickering emulsions stabilized by adsorption-crosslinking (C@FS-AC) and covalent bonding (C@FS-CB) immobilized lipase were then constructed, and their catalytic efficiency was evaluated via soybean oil hydrolysis as a model reaction. Their reusability, storage stability, catalytic stability, and the effects of metal ions/organic molecules on catalytic activity were also investigated. The results showed that the characterization results confirmed the successful immobilization of lipase on Fe3O4 nanoparticles. Compared with free lipase and immobilized lipase, the C@FS-AC Pickering emulsion and C@FS-CB Pickering emulsion catalytic systems enhanced the catalytic efficiency of soybean oil hydrolysis by 2.7 times and 8.6 times, as well as 4.1 times and 46.1 times, respectively, within 1 h of reaction. Both C@FS-AC and C@FS-CB Pickering emulsion catalytic systems exhibited excellent storage stability within 15 d; after 6 cycles of reuse, the immobilized lipase in the emulsions still retained 78.79% and 80.40% of their initial catalytic activity, respectively. The catalytic efficiency of the Pickering emulsions in oil hydrolysis was found to be dependent on the degree of oil saturation. The optimal reaction temperature for the catalytic systems was determined to be 35 ℃, with a relatively high catalytic efficiency observed in the first 25 h of the reaction. Low content of Fe3+ and gallic acid exerted a promoting effect, whereas high content showed an inhibitory effect; Cu2+ and curcumin inhibited the reaction, while vitamin E had no significant impact.In conclusion, the magnetic immobilized lipase Pickering emulsion catalytic systems demonstrated excellent stability and anti-interference capability, and exhibited high catalytic efficiency in oil-water two-phase catalysis. |
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