| 崔 洁,刘小芳,董 喆,薛 勇,薛长湖,王玉明.DHA-磷脂对肥胖小鼠脂质代谢的影响[J].中国油脂,2014,39(1):.[CUI Jie,LIU Xiaofang,DONG Zhe,XUE Yong,XUE Changhu,WANG Yuming.Effects of DHA-enriched phospholipids on lipid metabolism in diet-induced obese C57BL/6J mice model[J].China Oils and Fats,2014,39(1):.] |
| DHA-磷脂对肥胖小鼠脂质代谢的影响 |
| Effects of DHA-enriched phospholipids on lipid metabolism in diet-induced obese C57BL/6J mice model |
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| DOI： |
| 中文关键词: 二十二碳六烯酸 磷脂 肥胖 脂质代谢 |
| 英文关键词:docosahexaenoic acid phospholipids obesity lipid metabolism |
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| 摘要点击次数: 1492 |
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| 中文摘要: |
| 研究了二十二碳六烯酸-磷脂（DHA-磷脂）对实验性肥胖小鼠脂质代谢的调节作用及其机制。采用Folch法和硅胶柱层析法从鸢乌贼（文字Sthenoteuthis oualaniensis）卵中分离制备得到富含DHA的磷脂。C57BL/6J雄性小鼠随机分为3组：高脂模型组，1%大豆磷脂组，1%DHA-磷脂组。连续饲喂7周后，测定小鼠血清总胆固醇（TC）、甘油三酯（TG）、高密度脂蛋白胆固醇（HDL-C）含量，肝脏TC、TG、磷脂（PL）含量以及肝脏脂肪合成酶（FAS）、葡萄糖-6-磷酸脱氢酶（G6PDH）、苹果酸酶（ME）、磷脂酸磷酸酶（PAP）、肉毒碱棕榈酸转移酶（CPT）、过氧化物酶体β-氧化活力。结果显示，DHA-磷脂能极显著抑制小鼠体重（P＜0.01）和肾周脂肪（P＜0.01）及精周脂肪（P＜0.01）蓄积，降低血清TC（P＜0.01）和肝脏TG（P＜0.01）水平，抑制肝脏FAS（P＜0.01）、ME（P＜0.05）、PAP（P＜0.05）的活力，提高CPT（P＜0.01）和过氧化物酶体β-氧化（P＜0.05）的活力，且效果优于大豆磷脂。DHA-磷脂具有良好的减肥作用，能够通过抑制肝脏脂质合成，促进脂质分解，有效调节肥胖小鼠的脂质代谢。 |
| 英文摘要: |
| The effects of docosahexaenoic acid-enriched phospholipids （DHA-PL） on lipid metabolism in diet-induced obese C57BL/6J mice model were investigated. DHA-PL were extracted from Sthenoteuthis oualaniensis spawns by Folch method and purified by silica gel column chromatography. The mice were assigned to three groups randomly: high-fat model group, 1% soybean phospholipids group and 1% DHA-PL group. After seven weeks of feeding, the contents of triglycerides （TG）, total cholesterol （TC） and high density lipoprotein cholesterol （HDL-C） in serum and the contents of TC, TG and PL in liver of the mice were meassured, and the activities of hepatic fatty acid synthase（FAS）, malic enzyme（ME）, glucose-6-phosphate dehydrogenase（G6PDH）, carnitine palmitoyl transterase （CPT）, peroxisomal β-oxidation enzymes and phosphatidic acid phophyhydrolase （PAP） were determined. The results showed that DHA-PL inhibited the accumulations of body weight （P＜0.01）, perirenal adipose tissue（P＜0.01） and epididymal adipose tissue （P＜0.01） of mice significantly, reduced the contents of TC （P＜0.01） in serum and hepatic TG （P＜0.01）, inhibited the activities of FAS （P＜0.01）, ME （P＜0.05） and PAP （P＜0.05） in liver markedly, and enhanced the activities of CPT （P＜0.01） and peroxisomal β-oxidation enzymes （P＜0.05）. The effect of DHA-PL on improving the lipid metabolism was superior to that of soybean phospholipids. DHA-PL played a good role in weight loss, and it could promote decomposition of lipid through inhibiting synthesis of lipid in liver and regulate lipid metabolism of obese mice effectively. |
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