| 卢美欢,李利军,马英辉，等.响应面法优化终极腐霉生产EPA的发酵工艺[J].中国油脂,2015,40(9):.[LU Meihuan,LI Lijun,MA Yinghui，etc.Optimization of Pythium ultimum fermentation for EPA production by response surface methodology[J].China Oils and Fats,2015,40(9):.] |
| 响应面法优化终极腐霉生产EPA的发酵工艺 |
| Optimization of Pythium ultimum fermentation for EPA production by response surface methodology |
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| DOI： |
| 中文关键词: 响应面法 终极腐霉 EPA 发酵工艺 |
| 英文关键词:response surface methodology Pythium ultimum EPA fermentation process |
| 基金项目: |
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| 摘要点击次数: 718 |
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| 中文摘要: |
| 为进一步提高终极腐霉EPA产量，在初步优化发酵条件的基础上，通过Plackett-Burman和Box-Behnken实验设计优化终极腐霉生产EPA发酵工艺。获得的最优发酵工艺条件为：蔗糖8%，硝酸钾0.4%，酵母粉0.65%，磷酸氢二钠0.175%，硫酸镁0.065%，大豆油1.0%，起始pH 6.0，装液量50 mL/250 mL。在最优发酵工艺条件下，EPA产量达到541.61 mg/L，比初步优化产量（456.39 mg/L）提高了85.22 mg/L。 |
| 英文摘要: |
| In order to increase the EPA yield produced by Pythium ultimum, the fermentation process of Pythium ultimum for EPA production was optimized by Plackett-Burman design combined with Box-Behnken design on the basis of preliminary optimal fermentation conditions. The results showed that the optimal fermentation conditions were obtained as follows: sucrose 8%, KNO3 0.4%, yeast powder 0.65%, Na2HPO40.175%, MgSO40.065%, soybean oil 1.0%, initial pH 6.0, liquid volume in flask 50 mL/250 mL.Under the optimal conditions, EPA yield reached 541.61 mg/L, which increased by 85.22 mg/L compared with that of preliminary optimization （456.39 mg/L）. |
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