| 祝雨筱，王盈盈，金青哲，王兴国，王小三.酶法合成1，2-甘油二酯的工艺研究[J].中国油脂,2017,42(01):.[ZHU Yuxiao，WANG Yingying，JIN Qingzhe，WANG Xingguo，WANG Xiaosan.Enzymatic synthesis of 1,2-diacylglyceride[J].China Oils and Fats,2017,42(01):.] |
| 酶法合成1，2-甘油二酯的工艺研究 |
| Enzymatic synthesis of 1,2-diacylglyceride |
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| DOI： |
| 中文关键词: 1,2-甘油二酯 醇解 酶法合成 Lipozyme 435 |
| 英文关键词:1,2-diacylglyceride alcoholysis enzymatic synthesis Lipozyme 435 |
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| 中文摘要: |
| 以固定化脂肪酶Lipozyme 435为催化剂，在无溶剂体系下催化高油酸葵花籽油与无水乙醇发生醇解反应合成1,2-甘油二酯。通过单因素试验确定的最优反应条件为：高油酸葵花籽油与无水乙醇摩尔比1∶?50，加酶量6%（占底物总质量），反应温度50?℃，反应时间1 h。在最优反应条件下，1,2-甘油二酯得率可达88%。粗产物经两步结晶法提纯后，可获得纯度为100%的1,2-甘油二酯。 |
| 英文摘要: |
| The immobilized lipase Lipozyme 435 was used as catalyst to catalyze the alcoholysis of high oleic sunflower seed oil(HOSO) and absolute ethanol in a non-solvent system to synthesize 1,2-diacylglyceride(DAG). The optimal reaction conditions were obtained by single factor experiment as follows: molar ratio of HOSO to absolute ethanol 1∶?50, dosage of enzyme 6%(based on the total mass of substrates), reaction temperature 50?℃, reaction time 1 h. Under the optimal conditions, the yield of 1,2-DAG reached 88%.The purity of 1,2-DAG reached 100% after two-step solvent crystallization of crude product. |
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