| 黎殊，杨园园，付思源，等.在线固相萃取-高效液相色谱法（HPLC）测定煎炸用植物油中苯并(a)芘的含量[J].中国油脂,2017,42(10):.[LI Shu，YANG Yuanyuan，FU Siyuan，etc.Determination of benzo(a)pyrene content in frying vegetable oils by on-line solid phase extraction-high performance liquid chromatography[J].China Oils and Fats,2017,42(10):.] |
| 在线固相萃取-高效液相色谱法（HPLC）测定煎炸用植物油中苯并(a)芘的含量 |
| Determination of benzo(a)pyrene content in frying vegetable oils by on-line solid phase extraction-high performance liquid chromatography |
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| DOI： |
| 中文关键词: 苯并（a）芘 在线固相萃取 HPLC 煎炸用植物油 |
| 英文关键词:benzo(a)pyrene on-line solid phase extraction HPLC frying vegetable oil |
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| 中文摘要: |
| 建立了在线固相萃取-高效液相色谱法（HPLC）测定煎炸用植物油中苯并（a）芘（BaP）含量的方法。样品用异丙醇-环己烷(体积比1∶1)溶解，经在线固相富集柱净化浓缩后，C18色谱柱分离，荧光检测器进行检测。结果表明：BaP在0.2～10 ng/mL质量浓度范围内与峰面积呈现良好的线性关系，相关系数0.999 9，方法的检出限为0.50 μg/kg；在BaP加标量为5～15 ng范围内，BaP加标回收率为101%～106%，RSD为2.6%～3.2%。该方法具有操作简单、毒性低、耗时短、重复性好和准确度高等优点，适用于大批量煎炸用植物油中BaP的检测。 |
| 英文摘要: |
| A method for determining benzo(a)pyrene (BaP) content in frying vegetable oil by on-line solid phase extraction-high performance liquid chromatography was established. The samples were dissolved in isopropyl alcohol-cyclohexane(volume ratio 1∶1), cleaned up using on-line solid phase concentration column, and separated by C18column, then detected by fluorescence detector. The results showed that the BaP had good linear relationship in the range of 0.2-10 ng/mL, correlation coefficent was 0。999 9, and the limit of detection was 0.50 μg/kg. The recovery was 101%-106%, and RSD was 2.6%-3.2% with addition of 5-15 ng BaP in samples.This method had the items of simple operation, low toxicity, short time-consumption, good repeatability and accuracy, and could be applied in the detection of BaP in multitudinous frying vegetable oils. |
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