| 孟繁磊，宋志峰，牛红红，等.基于UPLC-MS-MS技术快速测定花生中4种黄曲霉毒素[J].中国油脂,2018,43(1):.[MENG Fanlei, SONG Zhifeng, NIU Honghong,etc.Rapid determination of four kinds of aflatoxins in peanut by UPLC-MS-MS[J].China Oils and Fats,2018,43(1):.] |
| 基于UPLC-MS-MS技术快速测定花生中4种黄曲霉毒素 |
| Rapid determination of four kinds of aflatoxins in peanut by UPLC-MS-MS |
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| DOI： |
| 中文关键词: UPLC-MS-MS 花生 黄曲霉毒素 快速测定 |
| 英文关键词:UPLC-MS-MS peanut aflatoxin rapid determination |
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| 摘要点击次数: 910 |
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| 中文摘要: |
| 建立了花生中4种黄曲霉毒素的超高液相色谱-三重四极杆串联质谱（UPLC-MS-MS）检测的方法。样品经80%乙腈-水溶液振荡超声提取后，采用多功能净化柱净化。待测物经Kinetex SB-C18色谱柱分离，甲醇-0.1%甲酸溶液为流动相，梯度洗脱，三重四级杆串联质谱多反应离子监测（MRM）方式检测，外标法定量。结果表明：4种黄曲霉毒素在各自的线性响应范围内线性关系良好，相关系数均大于0.999，方法的检出限为0.014~0.025 μg/kg，空白样品中加标回收率为72.25%~89.46%，RSD在4.01%~9.56%之间。该方法前处理简单快速、净化效果好、检出限低，适合花生中多种黄曲霉毒素的同时快速测定。 |
| 英文摘要: |
| A UPLC-MS-MS method for detecting four kinds of aflatoxins in peanut was established. The samples were extracted in 80% acetonitrile-water solution by ultrasonic vibration, and purified using a multi-functional cleanup column. The sample was separated by Kinetex SB-C18 chromatographic column, subjected to gradient elution using methanol-0.1% formic acid solution as the mobile phase, then detected by triple-quadrupole mass spectrometry with multiple reaction monitoring （MRM）, and analyzed quantitatively by the external standard method. The results suggested that four kinds of aflatoxins presented good linearity within their respectively linear response ranges, and the coefficient of correlation were above 0.999; the methods limit of detection was 0.014-0.025 μg/kg; the recoveries of standard addition for blank samples were 72.25%-89.46% with the RSDs 4.01%-956%. Characterized by simple and quick pre-treatment, good purification effect, and low limit of detection, this method could be used for simultaneously rapid determination of various aflatoxins in peanut. |
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