| 彭小东，王欢，李红洲，等.液相萃取-反相高效液相色谱法联用测定植物油中的苯并芘[J].中国油脂,2018,43(10):.[PENG Xiaodong, WANG Huan, LI Hongzhou，etc.Determination of benzopyrene in vegetable oil by liquid-phase extraction- RP-HPLC[J].China Oils and Fats,2018,43(10):.] |
| 液相萃取-反相高效液相色谱法联用测定植物油中的苯并芘 |
| Determination of benzopyrene in vegetable oil by liquid-phase extraction- RP-HPLC |
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| DOI： |
| 中文关键词: 苯并芘 反相高效液相色谱 液相萃取 |
| 英文关键词:benzopyrene RP-HPLC liquid-phase extraction |
| 基金项目: |
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| 摘要点击次数: 1017 |
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| 中文摘要: |
| 建立液相萃取-反相高效液相色谱法测定植物油中苯并芘的方法，以乙腈饱和的正己烷-正己烷饱和的乙腈作为萃取试剂，采用反相高效液相色谱仪对植物油中苯并芘的含量进行测定。所得线性方程Y=0.717 823X+0.159 747，相关系数r=0.999 99，线性范围在2~100 ng/mL，当乙腈饱和的正己烷与正己烷饱和的乙腈体积比为2∶?5时（即4 mL乙腈饱和的正己烷，10 mL正己烷饱和的乙腈），其回收率在82.62%，精密度为1.1%，检出限为0.4 μg/kg。该方法稳定、高效、检测成本低，适用于植物油中苯并芘的检测。 |
| 英文摘要: |
| A method for determination of benzopyrene(BaP) in vegetable oil by liquid-phase extraction-reversed phase high performance liquid chromatography （RP-HPLC）was established. With n-hexane saturated by acetonitrile-acetonitrile saturated by n-hexane as extraction solvent, the content of BaP in vegetable oil was determined by RP-HPLC.The results showed that there was linear relationship between peak area and BaP mass concentration in the range of 2-100 ng/mL, the linear equation was Y=0717 823X+0.159 747，and the correlation coefficient was 0.999 99.When the volume ratio of n-hexane saturated by acetonitrile to acetonitrile saturated by n-hexane was 2∶?5 (4 mL n-hexane saturated by acetonitrile, 10 mL acetonitrile saturated by n-hexane), the recovery rate was 82.62%, the precision was 1.1%, and the limit of detection was 0.4 μg/kg. The method was stable，efficient and low cost，and was suitable for the determination of BaP in vegetable oil． |
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