董庆霖,王瑶,邢向英,等.淡紫拟青霉TD16促进雨生红球藻生长和油脂合成的研究[J].中国油脂,2019,44(8):.[DONG Qinglin,WANG Yao,XING Xiangying,etc.Paecilomyces lilacinus TD16 promoting cell growth and lipid synthesis of Haematococcus pluvialis[J].China Oils and Fats,2019,44(8):.]
淡紫拟青霉TD16促进雨生红球藻生长和油脂合成的研究
Paecilomyces lilacinus TD16 promoting cell growth and lipid synthesis of Haematococcus pluvialis
  
DOI:
中文关键词:  雨生红球藻;淡紫拟青霉TD16;混合培养;生物量;油脂
英文关键词:Haematococcus pluvialis; Paecilomyces lilacinus TD16; mixed culture; biomass; lipid
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董庆霖,王瑶,邢向英,等  
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中文摘要:
      为提高雨生红球藻生物量和油脂产率,将浓度分别为1×107个/mL和1.36×107个/mL的雨生红球藻与淡紫拟青霉TD16以不同比例混合培养,确定5∶?1为最佳藻菌接种体积比。雨生红球藻与淡紫拟青霉TD16混合培养与雨生红球藻单独培养相比:生物量(0.92 g/L)和平均生长速率(62.30 mg/(L·d))分别提高24.32%和31.66%,倍增时间由4.26 d缩短至3.24 d;油脂产率(184.91 mg/L)和平均合成速率(13.21 mg/(L·d))分别提高45.59%和45.64%,脂肪酸组成中C16∶?0和C18∶?1分别上升20.56%和19.27%;最高叶绿素a和虾青素质量浓度达到3.89 mg/L和4.37 mg/L,分别提高20.81%和35.71%。说明淡紫拟青霉TD16与雨生红球藻混合培养能够促进雨生红球藻的生长,提高油脂产率。
英文摘要:
      In order to improve the biomass and lipid productivity of Haematococcus pluvialis, the H. pluvialis with the concentration of 1×107 cells/mL and Paecilomyces lilacinus TD16 with the concentration of 1.36×107 cells/mL were mixed and cultured in different proportions, and the optimal inoculation volume ratio was 5∶?1. Compared with individual culture, H. pluvialis in mixed culture:the biomass (0.92 g/L) and average growth rate (62.30 mg/(L·d)) increased by 24.32% and 31.66% respectively, while doubling time shortened from 4.26 d to 3.24 d; lipid productivity (184.91 mg/L) and the average lipid synthesis rate (13.21 mg/(L·d)) increased by 45.59% and 45.64% respectively, C16∶?0 and C18∶?1 contents increased by 20.56% and 19.27%, respectively; the highest mass concentrations of chlorophyll a (3.89 mg/L) and astaxanthin (4.37 mg/L) increased by 20.81% and 35.71% respectively. These results demonstrated that the mixed culture of P. lilacinus TD16 and H. pluvialis could promote the cell growth and lipid synthesis.
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