| 吴娟.高效液相色谱-荧光检测法快速检测植物油中的辣椒素和二氢辣椒素[J].中国油脂,2020,45(12):118~121.[WU Juan.Rapid determination of capsaicin and dihydrocapsaicin in vegetable oil byhigh performance liquid chromatography-fluorescence detection[J].China Oils and Fats,2020,45(12):118~121.] |
| 高效液相色谱-荧光检测法快速检测植物油中的辣椒素和二氢辣椒素 |
| Rapid determination of capsaicin and dihydrocapsaicin in vegetable oil byhigh performance liquid chromatography-fluorescence detection |
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| DOI：10.12166/j.zgyz.1003-7969/2020.12.022 |
| 中文关键词: 辣椒素 二氢辣椒素 植物油 高效液相色谱-荧光检测 |
| 英文关键词:capsaicin dihydrocapsaicin vegetable oil high performance liquid chromatography-fluorescence detection |
| 基金项目:国家自然科学基金项目（31371783） |
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| 摘要点击次数: 647 |
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| 中文摘要: |
| 以加标植物油为研究对象，通过优化前处理条件，用有机溶剂一步萃取植物油中的辣椒素和二氢辣椒素，再结合高效液相色谱和荧光检测器，建立了一种植物油中辣椒素、二氢辣椒素的快速检测方法。优化的前处理条件为：甲醇为萃取溶剂，萃取溶剂体积30 mL（油样质量30 g），萃取次数2次，每次萃取时间10 min。优化条件下，辣椒素、二氢辣椒素的回收率为81.08%～100.28%，日内、日间相对标准偏差分别在0.18%～1.92%和0.55%～5.14%范围内，两种目标物的检出限均为5 μg/kg。该方法前处理较为简单，省时省力、检测成本较低、实用性强，为植物油中辣椒素、二氢辣椒素提供了一种新的检测方法。 |
| 英文摘要: |
| A rapid method for determination of capsaicin and dihydrocapsaicin in vegetable oil by high performance liquid chromatography-fluorescence detection was established using organic solvent to extract capsaicin and dihydrocapsaicin from vegetable oil in one step through optimizing the pretreatment conditions. The pretreatment conditions of vegetable oil sample were optimized as follows: methanol used as extraction solvent, extraction solvent volume 30 mL (the oil sample mass 30 g), extraction times twice, and extraction time 10 min every time. Under the optimal conditions, the recovery rate of capsaicin and dihydrocapsaicin ranged from 81.08% to 100.28%, the intra-day and inter-day relative standard deviations ranged from 0.18% to 1.92% and from 0.55% to 5.14%, and the detection limit of the two targets was 5 μg/kg. The method was simple, time-saving, labor-saving, low-cost and practical, and could be used as a new method for determination of capsaicin and dihydrocapsaicin in vegetable oil. |
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