李满秀 任光明 王苗苗 孙 越 贾 佳.金纳米粒子荧光增强法测定食用油中PG的研究[J].中国油脂,2020,45(1):141~144.[LI Manxiu REN Guangming WANG Miaomiao SUN Yue JIA Jia.Determination of PG in edible oil based on fluorescence enhancing of gold nanoparticles[J].China Oils and Fats,2020,45(1):141~144.]
金纳米粒子荧光增强法测定食用油中PG的研究
Determination of PG in edible oil based on fluorescence enhancing of gold nanoparticles
  
DOI:10.12166/j.zgyz.1003-7969/2020.01.030
中文关键词:  金纳米粒子;没食子酸丙酯;荧光增强;食用油
英文关键词:gold nanoparticles; propyl gallate(PG); fluorescence enhancement;edible oi
基金项目:山西省1331工程重点学科建设计划项目(晋教财(2017)122);材料与计算化学山西省高等学校重点实验室基金
作者单位
李满秀 任光明 王苗苗 孙 越 贾 佳 忻州师范学院 化学系,山西 忻州 034000 
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中文摘要:
      采用聚乙烯亚胺作保护剂,利用THPC还原氯金酸制备金纳米粒子(AuNPs),该粒子溶液在375 nm激发下,在513 nm处有荧光发射。实验发现,AuNPs粒子于pH 7.8的磷酸盐缓冲介质中加入没食子酸丙酯(PG),体系的激发波长变为333 nm,发射波长变为380 nm,荧光蓝移且强度明显增强,由此建立了一种快速检测PG的新方法。考察了缓冲体系pH、反应时间、反应温度、AuNPs溶液浓度对PG测定的影响。结果表明,检测PG的最佳条件为:缓冲体系pH 7.8,反应时间20 min,反应温度25 ℃,AuNPs原液稀释10倍。在最佳条件下,PG与金纳米体系荧光强度在0.02~1.06 μg/mL范围内呈良好的线性关系,相关系数(R2)为0.999 3,检出限为9.7×10-3 μg/mL。该方法用于食用油中PG的检测,加标回收率为92.1%~104.1%。
英文摘要:
      The gold nanoparticles (AuNPs) were synthesized from chloroauric acid in aqueous solution with polyethyleneimine as protective agent and THPC as reductant. AuNPs showed a fluorescence emission at 513 nm under excitation of 375 nm. It was found that when the AuNPs were added with propyl gallate(PG)in a phosphate buffer medium with pH 7.8, the excitation wavelength of the system became 333 nm, and the intensity wavelength became 380 nm, which caused the gold nano-fluorescence blue shift and the intensity to be significantly enhanced.Thus a new method for determining PG rapidly was established. Different factors were studied, including the AuNPs concentration, buffer solution pH, reaction time and reaction temperature. The results showed that the optimal conditions for determining PG were obtained as follows:buffer solution pH 7.8, reaction time 20 min, reaction temperature 25 ℃ and AuNPs solution diluted ten times. Under the optimal conditions, the PG mass concentration had a better linear relationship with the AuNPs fluorescence intensity in the range of 0.02-1.06 μg/mL. The correlation coefficient (R2)was 0.999 3, and the detection limit was 9.7×10-3 μg/mL. The method was used to detect PG in edible oil, and its recovery rate of standard addition was 92.1%-104.1%.
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