| 肖怀秋.模拟人胃肠仿生消化对花生肽亚铁抗氧化活性的影响[J].中国油脂,2020,45(7):50~55.[XIAO Huaiqiu.Effect of simulate human gastrointestinal biomimetic digestion on antioxidant ability of peanut peptide-ferrous[J].China Oils and Fats,2020,45(7):50~55.] |
| 模拟人胃肠仿生消化对花生肽亚铁抗氧化活性的影响 |
| Effect of simulate human gastrointestinal biomimetic digestion on antioxidant ability of peanut peptide-ferrous |
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| DOI：10.12166/j.zgyz.1003-7969/2020.07.012 |
| 中文关键词: 花生肽亚铁 胃肠仿生消化 活性氧自由基 亚油酸氧化 脂质过氧化 |
| 英文关键词:peanut peptide-ferrous(PPF) gastrointestinal biomimetic digestion reactive oxygen species (ROS) linoleic acid oxidation liposome peroxidation |
| 基金项目:湖南省自然科学基金科教联合基金项目（2018JJ5022）；湖南省教育厅科研项目（18C1356） |
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| 中文摘要: |
| 为探讨花生肽亚铁在模拟人胃肠仿生消化条件下抗氧化活性变化规律，研究了仿生消化过程中表面疏水性、DPPH·清除率、O-2·清除率、·OH清除率、亚油酸氧化抑制活性和脂质过氧化抑制活性等指标变化。结果发现：模拟胃肠仿生消化后花生肽亚铁表面疏水性得到增强，仿生消化270 min时表面疏水性最大（（15.84±0.33）μg）；仿生消化增强了DPPH·清除活性，仿生消化300 min时，DPPH·清除率为（88.77±1.00）%，显著高于1.0 mg/mL VC（p<0.05）；胃肠仿生消化物O-2·和·OH清除活性分别显著低于1.0 mg/mL VC和0.6 mg/mL VC；胃肠仿生消化物具有较好的亚油酸氧化抑制作用，小肠仿生消化物抑制活性最高；仿生消化可增强脂质过氧化的保护效果，仿生消化330 min（质量浓度20 mg/L）时，脂质过氧化抑制效果最好，但显著低于VC对脂质过氧化的抑制作用（p<0.05）。试验结果表明，胃肠仿生消化可不同程度地增强花生肽亚铁的抗氧化活性。 |
| 英文摘要: |
| To investigate the changing rule of antioxidant activity of peanut peptide-ferrous (PPF) during simulated human gastrointestinal digestion (SHGD), the surface hydrophobicity, DPPH· scavenging rate, O-2 ·scavenging rate, ·OH scavenging rate, linoleic acid oxidation inhibitory activity and lipid peroxidation inhibitory activity of PPF during SHGD were studied. The results showed that SHGD enhanced the surface hydrophobicity, and the surface hydrophobicity of (15.84±0.33) μg was the highest at 270 min. SHGD enhanced the DPPH· scavenging capacity, the DPPH· scavenging rate was (88.77±1.00)% at 300 min, which was significantly higher than that of 1.0 mg/mL VC. The scavenging capacities on O-2· and ·OH of PPF gastrointestinal biomimetic digestive products were markedly weaker than that of 1.0 mg/mL VC and 0.6 mg/mL VC, respectively. The gastrointestinal biomimetic digestion product had better linoleic acid oxidation inhibition effect, and the intestinal biomimetic digestion products showed the highest inhibitory acitivity. Biomimetic digestion also enhanced the protection of lipid peroxidation, and the inhibition rate of lipid peroxidation was the highest at 330 min and 20 mg/L, which was significantly lower than that of VC. The results indicated that SHGD enhanced the antioxidant activity of PPF. |
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