| 韩坤坤.高温固态发酵制备豆粕多肽饲料中试试验探究[J].中国油脂,2020,45(7):73~77.[HAN Kunkun.Pilot-scale experiment on preparation of soybean meal polypeptide feed by high-temperature solid-state fermentation[J].China Oils and Fats,2020,45(7):73~77.] |
| 高温固态发酵制备豆粕多肽饲料中试试验探究 |
| Pilot-scale experiment on preparation of soybean meal polypeptide feed by high-temperature solid-state fermentation |
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| DOI：10.12166/j.zgyz.1003-7969/2020.07.016 |
| 中文关键词: 豆粕 高温固态发酵 中试试验 多肽 |
| 英文关键词:soybean meal high-temperature solid-state fermentation pilot-scale experiment polypeptide |
| 基金项目:江苏省重点研发计划(BE2016355)；镇江市重点研发计划（GZ2017006) |
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| 中文摘要: |
| 研究运用自主开发的一套高温发酵技术，采用实验室筛选驯化的一株优良高温芽孢杆菌，配合自主研制的滚筒固态发酵设备，将800 kg左右的豆粕与发酵种子液混合进行高温（55 ℃左右）固态发酵中试试验。结果表明：在发酵15 h左右高温发酵豆粕多肽含量提高近1倍，发酵52 h时粗蛋白含量提高5.90%，发酵后豆粕的尿素酶活性明显降低，16种氨基酸的含量均有不同程度的升高。试验证明使用该高温固态发酵技术与滚筒固态发酵设备进行发酵豆粕工业化生产具有可行性与潜力。 |
| 英文摘要: |
| Applying self-developed high-temperature fermentation technology，using a thermotolerant Bacillus strain screened in laboratory, a pilot-scale high-temperature solid-state fermentation experiment was conducted at about 55 ℃ with 800 kg soybean meal and fermented seed liquor mixed in self-developed drum fermentation tank. The results showed that the polypeptide content of high-temperature fermented soybean meal nearly doubled after fermenting for 15 h, while the crude protein content increased by 5.90%, the urease activity of fermented soybean meal decreased significantly, and the contents of 16 amino acids increased to some degree when fermented for 52 h. It was proved that the high-temperature solid-state fermentation technology applied in the drum fermentation tank was feasible and had potential for production of high-temperature fermented soybean meal in an industrial level. |
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