| 王朝仁1，黄忆婷1，杨柳1，王衍彬1，吴翠蓉1，薛锦松2.冷冻离心净化-高效液相色谱法测定植物油中苯并(a)芘含量[J].中国油脂,2021,46(5):.[WANG Chaoren1,HUANG Yiting1,YANG Liu1,WANG Yanbin1,WU Cuirong1,XUE Jinsong2.Determination of benzo (a) pyrene in vegetable oil by high performance liquid chromatography with freeze-centrifugation purification[J].China Oils and Fats,2021,46(5):.] |
| 冷冻离心净化-高效液相色谱法测定植物油中苯并(a)芘含量 |
| Determination of benzo (a) pyrene in vegetable oil by high performance liquid chromatography with freeze-centrifugation purification |
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| DOI： |
| 中文关键词: 关键词：苯并（a）芘 高效液相色谱 植物油 冷冻离心 净化 |
| 英文关键词:Key words：benzo (a) pyrene high performance liquid chromatography vegetable oil freeze-centrifugation purification |
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| 中文摘要: |
| 摘要：建立了冷冻离心净化-高效液相色谱测定植物油中苯并（a）芘含量的方法。样品经乙腈-丙酮（体积比4∶ 6）提取，采用冷冻离心净化，Kromasil C18柱分离，在乙腈-水（体积比9∶ 1）为流动相、流速1.0 mL/min、激发波长365 nm、发射波长406 nm条件下，采用荧光检测器检测。结果表明：苯并（a）芘在0.2~20 μg/L范围内线性关系良好（r=0.999 7）；植物油样品中苯并（a）芘低、中、高3个水平的平均加标回收率在81.3%~90.8%之间，相对标准偏差为1.35%~2.97%；检出限和定量限分别为0.13 μg/kg和0.5 μg/kg。该方法准确、简单，适于植物油中苯并（a）芘含量的测定。 |
| 英文摘要: |
| Abstract：A method was developed for the determination of benzo(a)pyrene in vegetable oil using freeze-centrifugation purification and high performance liquid chromatography. The sample was extracted with acetonitrile and acetone (volume ratio 4∶ 6) ,and then cleaned up by freeze-centrifugation. Benzo(a)pyrene was separated on a Kromasil C18 column,using a mobile phase consisting of acetonitrile and water (volume ratio 9∶ 1) at a flow rate of 1.0 mL/min. The excitation wavelength and emission wavelength were set at 365 nm and 406 nm respectively, and benzo (a) pyrene was detected with fluorescence detector. The results showed that the calibration curve showed good linearity in the range of 0.2-20 μg/L and the correlation coefficient was 0.999 7. The recoveries of benzo(a)pyrene in vegetable oil were in the range of 81.3%-90.8% at low, middle and high levels with RSD 1.35%-2.97%.The limit of detection(LOD) was 0.13 μg/kg and the limit of quantitation(LOQ) was 0.5 μg/kg. The established method was accurate, simple and suitable for the determination of benzo (a) pyrene in vegetable oil. |
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