| 王瑞丹.低强度亚临界水提取水溶性芝麻蛋白与多肽[J].中国油脂,2021,46(6):38~42.[WANG Ruidan.Extraction of water-soluble sesame protein and peptide by low strength subcritical water[J].China Oils and Fats,2021,46(6):38~42.] |
| 低强度亚临界水提取水溶性芝麻蛋白与多肽 |
| Extraction of water-soluble sesame protein and peptide by low strength subcritical water |
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| DOI： |
| 中文关键词: 高温芝麻粕 亚临界水 芝麻蛋白 多肽 |
| 英文关键词:high-temperature sesame meal subcritical water sesame protein peptide |
| 基金项目:中原科技创新领军人才项目（194200510014）；河南省科技攻关项目（202102110298）；国家特色油料产业技术体系（CARS-14） |
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| 中文摘要: |
| 以高温芝麻粕为原料，通过碱溶酸沉法提取芝麻蛋白，再利用低强度亚临界水提取芝麻蛋白中水溶性蛋白及多肽。通过单因素试验研究温度、pH、反应时间、液料比对水溶性蛋白及多肽得率的影响，再通过正交试验分别优化水溶性蛋白及多肽的提取工艺参数。结果表明：低强度亚临界水可显著提高水溶性蛋白及多肽的得率；低强度亚临界水提取水溶性蛋白的最优工艺参数为温度125 ℃、pH 1.5、反应时间30 min、液料比30∶ 1，在此条件下水溶性蛋白得率为85.015%；亚临界水提取多肽的最优工艺参数为温度125 ℃、pH 1.5、反应时间60 min、液料比50∶ 1，在此条件下多肽得率为40.537%。亚临界水提取率高，操作简便，为水溶性芝麻蛋白与多肽的提取提供参考依据。 |
| 英文摘要: |
| High-temperature sesame meal was used as the raw material, and the sesame protein was obtained by alkali-soluble and acid-precipitation, then low strength subcritical water was used to extract water-soluble protein and peptide. The effects of temperature, pH, reaction time and ratio of liquid to material on the yields of water-soluble protein and peptide were studied by single factor experiment, and the extraction process parameters of water-soluble protein and peptide were optimized by orthogonal experiment. The result showed that the yield of water-soluble protein and peptide could be significantly improved by low-strength subcritical water. The optimal process parameters for low strength subcritical water extraction of water-soluble protein were as follows: temperature 125 ℃, pH 1.5, reaction time 30 min,ratio of liquid to material 30∶ 1. Under the optimal conditions,the yield of water-soluble proteins was up to 85.015%. The optimal process parameters for subcritical water extraction of peptide were as follows: hydrolysis temperature 125 ℃, pH 1.5, reaction time 60 min, ratio of liquid to material 50∶ 1. Under the optimal conditions, the yield of peptide was up to 40537%. The extraction rate of subcritical water was high, the operation was simple, and it provided a reference for the extraction of water-soluble sesame protein and peptide. |
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