| 严俊安.3种色谱柱对多种脂肪酸甲酯分离效果比对[J].中国油脂,2021,46(6):130~136.[YAN Jun’an.Comparison of three chromatographic columns separating various fatty acid methyl esters[J].China Oils and Fats,2021,46(6):130~136.] |
| 3种色谱柱对多种脂肪酸甲酯分离效果比对 |
| Comparison of three chromatographic columns separating various fatty acid methyl esters |
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| DOI： |
| 中文关键词: 脂肪酸 脂肪酸甲酯 色谱柱 分离效果 |
| 英文关键词:fatty acid fatty acid methyl ester chromatographic column separation effect |
| 基金项目:深圳市卫生计生系统科研项目（SZFZ2017027, 201606043）；深圳市科创委基础研究项目（JCYJ2017 0307144652484）；国家卫生健康委员会食物成分监测项目；深圳市医疗卫生三名工程项目（SZSM201611017） |
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| 中文摘要: |
| 对比3种色谱柱对多种脂肪酸甲酯混标的分离效果，为快速准确地测定脂肪酸提供参考。分别建立HP-88、SP-2560、DB-23色谱柱分离37种脂肪酸甲酯混标的方法，并以此方法对13种反式脂肪酸甲酯、4种亚油酸甲酯和8种亚麻酸甲酯同分异构体进行分析。结果表明：3种色谱柱分离37种脂肪酸甲酯所需的时间分别为47、35、25 min，色谱峰间的分离度均大于1.0；3种色谱柱均能将顺反异构的单不饱和脂肪酸甲酯和亚油酸甲酯同分异构体分离开，而对于亚麻酸甲酯同分异构体和双键在不同位置的异构体，HP-88分离效果优于SP-2560和DB-23，但均不满足单独定量的要求。在实际样品分析时，可根据样品中脂肪酸种类选择合适的色谱柱，避免样品色谱峰的重叠。 |
| 英文摘要: |
| The results of three chromatographic columns separating various fatty acid methyl esters (FAME) standards were compared for the reference of detecting fatty acids quickly and accurately. The methods of separating 37 FAME standards with HP-88, SP-2560 and DB-23 were established and used to analyze 13 trans FAME standards, 4 isomers of linoleic acid methyl ester and 8 isomers of linolenic acid methyl ester. The results showed that the analysis time of three columns separating 37 FAME was 47, 35 min and 25 min respectively, and all of the resolutions were greater than 1.0. The cis-trans monounsaturated fatty acid methyl ester and linoleic acid methyl ester isomer could be separated by the three columns. HP-88 was better than SP-2560 and DB-23 in the separation of linolenic acid methyl ester and isomers of fatty acid with double bonds at different positions, but neither of them met the requirements of quantitative analysis. Suitable chromatographic columns could be selected according to the types of fatty acids in the samples to avoid the overlapping of chromatographic peaks in actual sample analysis. |
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