李世磊.酶法脱胶在大豆油脱胶中的应用[J].中国油脂,2021,46(10):1~5.[LI Wanping.Application of enzymatic degumming on soybean oil degumming[J].China Oils and Fats,2021,46(10):1~5.]
酶法脱胶在大豆油脱胶中的应用
Application of enzymatic degumming on soybean oil degumming
  
DOI:
中文关键词:  酶法脱胶  PLA1  PLC  单酶脱胶  双酶脱胶  得油率  含磷量
英文关键词:enzymatic degumming  PLA1  PLC  single enzyme degumming  double enzymes degumming  oil yield  phosphorus content
基金项目:“十三五”国家重点研发计划资助项目(2016 YFD0401405)
作者单位
李世磊 中粮营养健康研究院有限公司 营养健康与食品安全北京市重点实验室老年营养食品研究北京市工程实验室 北京102209 
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中文摘要:
      研究了PLA1酶法脱胶的影响因素,确定了实验室小试条件下PLA1酶法脱胶最佳工艺参数;并采用PLA1、PLC单酶脱胶以及PLC耦联PLA1双酶脱胶3种脱胶方式在不同批次大豆毛油上进行了小试验证。结果表明:PLA1酶法脱胶最佳工艺条件为酶添加量20 mg/kg、水添加量3%、搅拌速度500 r/min、反应温度55 ℃、反应时间2 h,在此条件下大豆毛油含磷量降至10 mg/kg以内;相对于水化脱胶,PLA1单酶脱胶得油率提升0.74~0.91个百分点,PLC单酶脱胶得油率提升0.70~0.84个百分点,双酶脱胶得油率提升1.34~1.89个百分点。经中试生产验证,PLA1和PLC单酶脱胶与水化脱胶相比得油率分别提升0.63个百分点和0.91个百分点。
英文摘要:
      The influencing factors of PLA1 enzymatic degumming were studied, and the optimal laboratory parameters of PLA1 enzymatic degumming were determined. Three degumming methods of PLA1, PLC and PLC combined with PLA1 double enzymes were tested on different batches of crude soybean oil. The results showed that the optimal conditions for the PLA1 enzymatic degumming were obtained as follows: enzyme dosage 20 mg/kg, water dosage 3%, stirring speed 500 r/min, reaction temperature 55 ℃ and reaction time 2 h. Under these conditions, the phosphorus content of soybean oil decreased to within 10 mg/kg. The oil yield increased respectively 0.74-0.91 percentage points by PLA1 enzymatic degumming, 0.70-0.84 percentage points by PLC enzymatic degumming, 1.34-1.89 percentage points by PLC combined with PLA1 enzymatic degumming. The pilot production showed that the oil yield of PLA1 and PLC single enzymes degumming increased by 063 percentage points and 0.91 percentage points, respectively compared with water deyumming.
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