| 于姮梅.九香虫油脂提取条件优化及体外抗乳腺癌活性研究[J].中国油脂,2022,47(2):34~38.[YU Hengmei.Optimization of extraction conditions of Aspongopus chinensis Dallas oil and its anti-breast cancer activity in vitro[J].China Oils and Fats,2022,47(2):34~38.] |
| 九香虫油脂提取条件优化及体外抗乳腺癌活性研究 |
| Optimization of extraction conditions of Aspongopus chinensis Dallas oil and its anti-breast cancer activity in vitro |
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| DOI： |
| 中文关键词: 九香虫 九香虫油脂 索氏抽提法 抗乳腺癌活性 |
| 英文关键词:Aspongopus chinensis Dallas Aspongopus chinensis Dallas oil Soxhlet extraction method anti-breast cancer activity |
| 基金项目:贵州省科技支撑计划项目（黔科合支撑\[2021\]一般180） |
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| 中文摘要: |
| 为了促进九香虫油脂的开发，采用单因素实验和正交实验对索氏抽提法提取九香虫油脂的工艺条件进行了优化，并采用CCK-8法检测九香虫油脂对小鼠乳腺癌细胞4T1和人乳腺癌细胞HCC1937增殖的影响。结果表明：九香虫油脂的最佳提取条件为液料比6∶ 1、提取温度90 ℃、提取时间3 h，在此条件下九香虫油脂提取率为（44.270±1.679）%；4、8、12、16 mg/mL九香虫油脂作用24 h，均能抑制两种乳腺癌细胞的增殖，且对于人乳腺癌细胞HCC1937的作用效果优于小鼠乳腺癌细胞4T1；另外，九香虫油脂处理导致两种乳腺癌细胞形态及数量均发生明显变化。综上，九香虫油脂对乳腺癌细胞具有显著抑制作用。 |
| 英文摘要: |
| In order to promote the development of the oil from Aspongopus chinensis Dallas, single factor experiment and orthogonal experiment were used to optimize the extraction conditions of A. chinensis Dallas oil by Soxhlet extraction method. Meanwhile, CCK-8 method was used to detect the effects of the extracted oil on the proliferation of mouse breast cancer cells 4T1 and human breast cancer cells HCC1937. The results showed that the optimal extraction conditions of A. chinensis Dallas oil were obtained as follows: liquid-solid ratio 6∶ 1, extraction temperature 90 ℃, extraction time 3 h. Under the optimal conditions, the extraction rate of oil was (44.270±1.679)%. The A. chinensis Dallas oil with the mass concentration of 4, 8, 12, 16 mg/mL treating for 24 h could inhibit the proliferation of two types of breast cancer cells, and the effect on human breast cancer cells HCC1937 was better than that on mouse breast cancer cells 4T1. Moreover, the morphology and quantity of the two types of cells were significantly changed by A. chinensis Dallas oil treatment. In summary, A. chinensis Dallas oil obtained has a significant inhibitory effect on breast cancer cells 4T1 and HCC1937 in vitro. |
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