| 高盼1,2,3，张慧慧1，王澍3,4，杨歆萌1，胡传荣1，何东平1,3，张晖5,6,张跃进6，王兴国1,5,6.酶法辅助制备核桃蛋白的工艺优化及超滤回收核桃蛋白[J].中国油脂,2022,47(10):.[GAO Pan1,2,3, ZHANG Huihui1, WANG Shu3,4, YANG Xinmeng1, HU Chuanrong1, HE Dongping1,3, ZHANG Hui5,6, ZHANG Yuejin6, WANG Xingguo1,5,6.Optimization of enzymatic-assisted preparation of walnut protein and ultrafiltration recovery of walnut protein[J].China Oils and Fats,2022,47(10):.] |
| 酶法辅助制备核桃蛋白的工艺优化及超滤回收核桃蛋白 |
| Optimization of enzymatic-assisted preparation of walnut protein and ultrafiltration recovery of walnut protein |
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| DOI： |
| 中文关键词: 核桃蛋白 纤维素酶 醋酸纤维素膜 酶法辅助 超滤回收 |
| 英文关键词:walnut protein cellulase cellulose acetate membrane enzymatic assist ultrafiltration recovery |
| 基金项目: |
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| 中文摘要: |
| 为提高核桃蛋白得率，分别探索了以碱性蛋白酶、纤维素酶和α-淀粉酶为辅助酶，碱溶酸沉法制备核桃蛋白的工艺，采用超滤工艺回收酸沉废液中核桃蛋白，比较醋酸纤维素（CA）膜、聚醚砜树脂（PES）膜和聚偏氟乙烯（PVDF）膜3种不同超滤膜的回收效率。结果表明，酶法辅助碱溶酸沉法制备核桃蛋白的最佳工艺条件为以纤维素酶为辅助酶、酶解时间60 min、酶解pH 3.6、酶添加量0.5%、酶解温度37℃，在最佳工艺条件下核桃蛋白得率为84.11%。CA膜能显著提高核桃蛋白得率，在原料液质量浓度0.363 mg/mL、原料液温度30℃的条件下，CA膜的截留率可达92.33%，核桃蛋白得率可达87.64%。 |
| 英文摘要: |
| In order to improve the yield of walnut protein, alkaline protease, cellulase and α-amylase were used as auxiliary enzyme to extract walnut protein by alkali-solution and acid-precipitation, walnut protein from acid precipitation waste water was recovered by ultrafiltration process, and the recovery efficiency of cellulose acetate (CA) membrane, polyethersulfone resin (PES) membrane and polyvinylidene fluoride （PVDF） membrane on walnut protein was compared. The results showed that the optimal preparation conditions of walnut protein were obtained as follows: with cellulase as auxiliary enzyme, enzymatic hydrolysis time 60 min, enzymatic hydrolysis pH 3.6, dosage of enzyme 0.5%, and enzymatic hydrolysis temperature 37℃.Under the optimal conditions,the yield of walnut protein was 84.11%. CA membrane could significantly improve the yield of walnut protein, under the conditions of mass concentration of feed solution 0.363 mg/mL and temperature of feed solution 30℃, the rejection rate of CA membrane could reach 92.33%，and the yield of walnut protein was 87.64%. |
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