| 李明丹1，朱恒1，王凯1，姜德建1,2.两种不同DHA含量的精制鱼油
对小鼠记忆改善作用比较[J].中国油脂,2025,50(5):.[LI Mingdan1, ZHU Heng1, WANG Kai1, JIANG Dejian1,2.Comparison of the memory-improving effects of two refined fish oils with different DHA contents on mice[J].China Oils and Fats,2025,50(5):.] |
| 两种不同DHA含量的精制鱼油
对小鼠记忆改善作用比较 |
| Comparison of the memory-improving effects of two refined fish oils with different DHA contents on mice |
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| DOI：10.19902/j.cnki.zgyz.1003-7969.240107 |
| 中文关键词: 鱼油 二十二碳六烯酸（DHA） 改善记忆 跳台实验 避暗实验 水迷宫实验 |
| 英文关键词:fish oil docosahexaenoic acid (DHA) improving memory jumping test dark avoidance test water maze test |
| 基金项目:湖南省药物非临床研究科技创新创业团队（2021） |
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| 中文摘要: |
| 旨在为鱼油的临床应用提供参考，以 DHA标注含量分别为85%、40%的精制鱼油为试样，将雌性ICR小鼠随机分为阴性对照组，鱼油（DHA 85%）低、中、高（0.2、0.4、0.6 g/kg）剂量正常组，鱼油（DHA 40%）低、中、高（0.2、0.4、0.6 g/kg）剂量正常组，模型对照组，鱼油（DHA 85%）低、中、高（0.2、0.4、0.6 g/kg）剂量模型组，鱼油（DHA 40%）低、中、高（0.2、0.4、0.6 g/kg）剂量模型组，按5 mL/kg经口灌胃，每日一次，连续30 d,末次给样后从阴性对照组及各剂量正常组中分别取10只小鼠直接进行跳台、避暗、水迷宫实验，从模型对照组及各剂量模型组中分别取10只小鼠于训练前腹腔注射东莨菪碱造模后进行跳台、避暗、水迷宫实验。结果表明：与阴性对照组相比，鱼油（DHA 85%）高剂量、鱼油（DHA 40%）高剂量正常组小鼠在跳台实验重测验阶段潜伏期明显延长（p≤0.05），鱼油（DHA 85%）中剂量、鱼油（DHA 40%）高剂量正常组小鼠在避暗实验消退阶段潜伏期明显延长（p≤0.05）；与模型对照组相比，鱼油（DHA 85%）中剂量模型组小鼠在跳台实验重测验阶段潜伏期明显延长（p≤0.05）、错误反应小鼠比例明显降低（p≤0.05），鱼油（DHA 85%）低剂量模型组小鼠在避暗实验重测验阶段和消退阶段的潜伏期明显延长（p≤0.05）, 鱼油（DHA 40%）高剂量模型组小鼠在避暗实验训练阶段的错误次数明显降低（p≤0.05），鱼油（DHA 85%）、鱼油（DHA 40%）低、中、高剂量模型组小鼠在水迷宫实验的训练和测试阶段到达终点的总时间明显缩短（p≤0.05或p≤0.01），鱼油（DHA 85%）中、高剂量及鱼油（DHA 40%）高剂量模型组小鼠在水迷宫实验的训练和测试阶段总错误次数均明显减少（p≤0.05），鱼油（DHA 85%）低、中、高剂量及鱼油（DHA 40%）中剂量模型组小鼠在水迷宫实验的训练和测试阶段到达终点总小鼠数量占比均明显增加（p≤0.05或p≤0.01）；与鱼油（DHA 40%）高剂量模型组相比，鱼油（DHA 85%）中剂量模型组小鼠在跳台实验重测验阶段的潜伏期明显延长（p≤0.05），错误反应小鼠比例明显降低（p≤0.05），鱼油（DHA 85%）低剂量模型组小鼠在避暗实验消退阶段的潜伏期明显延长（p≤0.05）。综上，鱼油（DHA 85%）、鱼油（DHA 40%）具有改善小鼠记忆作用，且鱼油（DHA 85%）效果优于鱼油（DHA 40%）。 |
| 英文摘要: |
| To provide a reference for the clinical application of fish oil, refined fish oils with DHA-labeled contents of 85% and 40% were used as test samples, female ICR mice were randomly divided into the following groups: a negative control (NC) group, a normal group with low-, medium-, and high-doses of DHA-labeled fish oil (85% and 40%) at 0.2, 0.4 g/kg, and 0.6 g/kg, a model control (MC) group, a model group with low-, medium-, and high-doses of DHA-labeled fish oil (85% and 40%) at 0.2, 0.4 g/kg, and 0.6 g/kg, and the mice were administered 5 mL/kg of the fish oil orally once a day for 30 d. After the last administration, 10 mice from the NC group and each dose normal group were directly subjected to the jumping test, dark avoidance test, and water maze test, while the model groups were tested with jumping test, dark avoidance test, and water maze test after intraperitoneal injection of scopolamine to induce the model. The results showed that compared with the NC group, the high-dose normal group of fish oil (DHA 85% and 40%) had significantly longer retest phase latency in the jumping test (p≤0.05), and the medium-dose normal group of fish oil (DHA 85%) and the high-dose normal group of fish oil (DHA 40%) had significantly longer extinction phase latency in dark avoidance test (p≤0.05). Compared with MC group, the medium-dose model group of fish oil (DHA 85%) had significantly longer retest phase latency (p≤0.05) and a significantly lower error rate of animals (p≤0.05) in the jumping test, the low-dose model group of fish oil (DHA 85%) had significantly longer retest and extinction phase latency in the dark avoidance test (p≤0.05), the errors number of the high-dose model group of fish oil (DHA 40%) in the dark avoidance test training stage was significantly reduced (p≤0.05). In the water maze test, compared with MC group, the total time to reach the end in the low-, medium-, and high-dose model groups of fish oil (DHA 85%) and fish oil (DHA 40%) at the training and test stage decreased significantly (p≤0.05 or p≤0.01), the total number of errors at the training and test stage in the medium- and high-dose model groups of fish oil (DHA 85%) and the high-dose model groups of fish oil (DHA 40%) reduced significantly (p≤0.05), and the proportion of the total number of animals reaching the end at the training and test stage in the low-, medium- and high-dose model groups of fish oil (DHA 85%) and the medium-dose model groups of fish oil (DHA 40%) increased significantly (p≤0.05 or p≤0.01). In the model group, the latency of medium-dose fish oil (DHA 85%) in the retest stage of jumping test was significantly longer than that in the high-dose of DHA 40%(p≤0.05), and the proportion of mice with false response was significantly lower than that in the high-dose of fish oil (DHA 40%) (p≤0.05), and the latency of low-dose of fish oil (DHA 85%) in dark avoidance test was significantly longer than that of high dose of fish oil (DHA 40%)(p≤0.05). In summary, fish oil (DHA 85%) and fish oil (DHA 40%) can improve the memory of mice, and the effect of fish oil (DHA 85%) is better than that of fish oil (DHA 40%). |
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