臧佳辰,陶 莎,薛璟怡,等.火麻油脂肪酶水解条件的优化研究[J].中国油脂,2013,38(7):.[ZANG Jiachen,TAO Sha,XUE Jingyi,etc.Optimization of enzymatic hydrolysis conditions of hemp seed oil[J].China Oils and Fats,2013,38(7):.] |
火麻油脂肪酶水解条件的优化研究 |
Optimization of enzymatic hydrolysis conditions of hemp seed oil |
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DOI: |
中文关键词: 火麻油 脂肪酶 水解条件 |
英文关键词:hemp seed oil lipase hydrolysis condition |
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中文摘要: |
以优化火麻油酶水解条件为目的,Mucor javanicus脂肪酶作为实验用酶对火麻油进行水解研究,在反应时间12 h、反应温度45℃条件下,通过响应面实验对反应体系的水油比、酶添加量、pH进行优化,得到反应最优条件为:水油比15∶1,酶添加量952 U/g,pH 6.62。在此条件下火麻油水解率为96.50%。 |
英文摘要: |
Hemp seed oil was hydrolyzed by lipase Mucor javanicus. Based on the reaction time 12 h and reaction temperature 45℃, the ratio of water to oil, lipase dosage and pH were optimized by response surface methodology. The results indicated that the optimal reaction conditions were as follows:ratio of water to oil 15∶1, lipase dosage 952 U/g, pH 6.62. Under these conditions, the hydrolysis rate of hemp seed oil was 96.50%. |
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