| 邱丰艳,丁 力,曹红云.高效液相色谱法测定油脂中β-谷甾醇的含量[J].中国油脂,2014,39(7):.[QIU Fengyan,DING Li,CAO Hongyun.Determination of β-sitosterol content of oil mixture by HPLC[J].China Oils and Fats,2014,39(7):.] |
| 高效液相色谱法测定油脂中β-谷甾醇的含量 |
| Determination of β-sitosterol content of oil mixture by HPLC |
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| DOI： |
| 中文关键词: HPLC β-谷甾醇 油脂混合物 |
| 英文关键词:HPLC β-sitosterol oil mixture |
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| 中文摘要: |
| 高效液相色谱法准确、高效测定油脂混合体系中β-谷甾醇含量的方法，并对油脂混合物中β-谷甾醇的提取工艺进行了优化。采用C18反相色谱柱对β-谷甾醇含量进行测定，流动相为甲醇-水（体积比98.5∶1.5），检测波长210 nm。结果表明：β-谷甾醇在25～500 μg/mL范围内，质量浓度与峰面积呈良好的线性关系(R2=0.999 8)，仪器检出限为7.5 μg/mL，相对标准偏差（RSD）为0.85%；油脂混合物中β-谷甾醇的最佳提取工艺条件为提取次数4次，离心转速2 000 r/min，皂化温度80℃，皂化时间210 min；在最佳提取工艺条件下β-谷甾醇的回收率为93.44%～96.48%，RSD为1.70%。 |
| 英文摘要: |
| The accurate and efficient method for determing β-sitosterol content of oil mixture by HPLC was established,and the extraction process of β-sitosterol from oil mixture was optimized.C18 reverse-phase chromatography column was used for the determination of β-sitosterol content under the conditions of mobile phase methanol - water (volume ratio 98.5∶1.5 ) and detection wavelength 210 nm. The results showed that the mass concentration of β-sitosterol had a good linear relationship with peak area in the range of 25-500 μg/mL. The correlation coefficient, the instrumental limit of detection and the relative standard deviation (RSD) were 0.999 8, 7.5 μg/mL and 0.85% respectively. The optimal extraction conditions of β-sitosterol from oil mixture were obtained as follows: extraction times four, centrifugal speed 2 000 r/min, saponification temperature 80℃and saponification time 210 min. Under these conditions,the recovery rate and RSD of β-sitosterol were 93.44%-96.48% and 1.70%, respectively. |
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