| 张康逸,张薇薇,纵 伟，等.溶血磷脂酰乙醇胺硅胶柱色谱纯化技术研究[J].中国油脂,2015,40(7):.[ZHANG Kangyi,ZHANG Weiwei,ZONG Wei,etc.Purification of lysophosphatidylethanolamine using silica gel column chromatography[J].China Oils and Fats,2015,40(7):.] |
| 溶血磷脂酰乙醇胺硅胶柱色谱纯化技术研究 |
| Purification of lysophosphatidylethanolamine using silica gel column chromatography |
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| DOI： |
| 中文关键词: 磷脂酰乙醇胺 溶血磷脂酰乙醇胺 硅胶柱色谱 纯化 |
| 英文关键词:phosphatidylethanolamine lysophosphatidylethanolamine silica gel column chromatography purification |
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| 摘要点击次数: 1003 |
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| 中文摘要: |
| 探讨了溶血磷脂酰乙醇胺（LPE）硅胶柱色谱纯化的可行性。通过选择，确定以硅胶为固定相、氯仿-甲醇（体积比2∶1）为洗脱液，进行LPE纯化工艺优化。得到的最优工艺条件为：上样量1∶[KG-2mm]40（样品质量与固定相质量比），上样液质量浓度30 mg/mL，洗脱流速2.5 mL/min；在最佳工艺条件下，最终LPE产品纯度为99.05%，回收率为88.69%。研究为进一步探讨工业化制备LPE提供了基础数据和理论支撑。 |
| 英文摘要: |
| The feasibility of purification of lysophosphatidylethanolamine (LPE) using silica gel column chromatography was studied. Through selection, choosing silica gel as stationary phase and chloroform- methanol(volume ratio 2∶1)as eluent, the purification process of LPE was optimized as follows: sample amount 1∶40 (mass ratio of sample to stationary phase), mass concentration of sample 30 mg/mL, flow rate of eluent 2.5 mL/min; under these conditions,the purity and recovery rate of LPE were 99.05% and 88.69%,respectively. The study provided fundamental data and theoretical support for the further industrial preparation of LPE. |
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