| 陆继源，王小三，金青哲，王兴国.响应面优化酶法酯交换催化合成中长链结构甘三酯[J].中国油脂,2017,42(5):.[LU Jiyuan, WANG Xiaosan, JIN Qingzhe, WANG Xingguo.Optimization of Lipozyme 435-catalyzed synthesis of medium-and long-chain triglycerides using response surface methodology[J].China Oils and Fats,2017,42(5):.] |
| 响应面优化酶法酯交换催化合成中长链结构甘三酯 |
| Optimization of Lipozyme 435-catalyzed synthesis of medium-and long-chain triglycerides using response surface methodology |
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| DOI： |
| 中文关键词: 中长链结构甘三酯 Lipozyme 435 酯交换 响应面 甘三酯构型 |
| 英文关键词:medium-and long-chain triglycerides Lipozyme 435 interesterification response surface methodology triglycerides structure |
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| 中文摘要: |
| 采用响应面法对脂肪酶Lipozyme 435在无溶剂体系中催化中链甘三酯（MCT）和大豆油酯交换反应合成中长链甘三酯的反应条件进行优化,并采用超高压液相色谱-飞行时间质谱在正离子模式下对反应产物的甘三酯构型进行分析。结果表明：反应温度和反应时间对中长链甘三酯含量具有极显著性影响(P<0.01)。最佳反应条件为大豆油与MCT质量比60∶?40，反应时间4.36 h，反应温度89 ℃，加酶量(以底物质量计)5.5%。在此条件下，中长链甘三酯含量达到84.15%。CyCyL、CyCyLn、LOCy、LPCy、OOCy和LOCa为反应产物的主要甘三酯构型。 |
| 英文摘要: |
| The synthesis conditions of medium- and long-chain triglycerides by Lipozyme 435-catalyzed interesterification of soybean oil and medium-chain triglycerides in a solvent-free system was optimized by response surface methodology. The triglycerides structure of the product was analyzed by ultra-performance liquid chromatography (UPLC) coupled with quadrupole time of flight mass spectrometry under positive ion model. The results showed that reaction temperature and reaction time had extremely significant influence on medium-and long-chain triglycerides content. The optimal reaction conditions were obtained as follows： mass ratio of soybean oil to medium-chain triglycerides 60∶?40, reaction time 4.36 h, reaction temperature 89?℃, and enzyme dosage 5.5%(based on the mass of substrate). Under these conditions, medium- and long-chain triglycerides content was 84.15%. The main triglycerides in the products were CyCyL, CyCyLn, LOCy, LPCy, OOCy and LOCa. |
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