| 赵琦琰，杨瑞金，刘军军，等.直接进样HPLC-ELSD法测定花生中磷脂含量[J].中国油脂,2018,43(3):.[ZHAO Qiyan, YANG Ruijin, LIU Junjun，etc.Determination of phospholipids in peanut by direct-injection HPLC-ELSD[J].China Oils and Fats,2018,43(3):.] |
| 直接进样HPLC-ELSD法测定花生中磷脂含量 |
| Determination of phospholipids in peanut by direct-injection HPLC-ELSD |
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| DOI： |
| 中文关键词: 花生 磷脂 HPLC-ELSD 定量分析 |
| 英文关键词:peanut phospholipids HPLC-ELSD quantitative analysis |
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| 中文摘要: |
| 建立了直接进样高效液相色谱-蒸发光检测器（HPLC-ELSD）法测定花生中磷脂酰胆碱（PC）、磷脂酰乙醇胺（PE）、磷脂酰肌醇（PI）和磷脂酸（PA）的方法。采用改良Folch方法提取样品总脂肪，然后将提取出的总脂肪直接溶于流动相进行液相色谱分析，以L-Si型正相硅胶色谱柱（250 mm×4.6 mm，5 μm）为固定相，以正己烷-异丙醇（体积比2∶?3）为流动相A，正己烷-异丙醇-25 mmol/L乙酸铵溶液（体积比3.6∶?5.4∶?1）为流动相B进行二元梯度洗脱，蒸发光检测器检测。PC、PE、PI和PA 4种磷脂色谱峰面积与其质量浓度的线性相关系数（R2）大于0.995，4种磷脂的平均回收率在93.59%~108.57%之间，RSD均小于10%。该方法具有重现性好、稳定性高、前处理简单的特点，可应用于磷脂的分离检测。 |
| 英文摘要: |
| A method was established to determine phosphatidylcholine（PC），phosphatidyl ethanolamine（PE），phosphatidylinositol（PI）and phosphatidate（PA） in peanut by direct-injection HPLC-ELSD. A modified Folch method was used to extract the total fat and then the extract dissolved in the mobile phase was taken directly into HPLC. The HPLC method was performed on a column of L-Si (250 mm×4.6 mm, 5 μm) by gradient elution using hexane-isopropanol （volume ratio 2∶?3）and hexane-isopropanol- 25 mmol/L ammonium acetate（volume ratio 3.6∶?5.4∶?1）as the mobile phases and detected by evaporative light-scattering detector. The correlation coefficient of standard curve for mass concentration and peak area of each phospholipid in linearity range was above 0.995, as well as the average recovery rates of the four phospholipids in peanut were in the ranges of 93.59%-108.57% with the relative standard deviations(RSDs)less than 10%. The method had advantages of good reproducibility, high stability and simple pretreatment and it should be used for separation and determination of phospholipids. |
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