| 陈程，张存劳，罗国平，等.超声波辅助纤维素酶提取牡丹籽饼中多糖及其清除自由基活性研究[J].中国油脂,2018,43(4):.[CHEN Cheng,ZHANG Cunlao, LUO Guoping,etc.Ultrasound-assisted cellulose extraction of polysaccharide from peony seed cake and its free radical scavenging activity[J].China Oils and Fats,2018,43(4):.] |
| 超声波辅助纤维素酶提取牡丹籽饼中多糖及其清除自由基活性研究 |
| Ultrasound-assisted cellulose extraction of polysaccharide from peony seed cake and its free radical scavenging activity |
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| DOI： |
| 中文关键词: 牡丹籽饼 多糖 自由基清除能力 超声波辅助提取 PB试验 BBD响应面法 |
| 英文关键词:peony seed cake polysaccharide free radical scavenging activity ultrasound-assisted extraction PB design BBD response surface methodology |
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| 中文摘要: |
| 采用超声波辅助纤维素酶提取牡丹籽饼中多糖。在单因素试验的基础上，采用PB设计对影响多糖提取量的9个因素（pH、加酶量、酶解时间、酶解温度、超声时间、超声功率、超声温度、液料比、粒度）进行显著性分析。通过BBD响应面法优化最佳提取工艺条件。采用清除DPPH自由基活性评价牡丹籽饼中多糖的抗氧化能力。结果表明，牡丹籽饼中多糖的最佳提取工艺条件为：加酶量0.45%，酶解时间60 min，酶解温度45?℃，pH 4.5，超声时间19 min，超声功率300 W，超声温度40?℃，液料比19∶?1，粒度60目。在最佳工艺条件下，牡丹籽饼中多糖提取量为196.87 mg/g。牡丹籽多糖具有一定DPPH自由基清除能力，但弱于VC，其IC50值为31.19 μg/mL。 |
| 英文摘要: |
| The ultrasound-assisted cellulose extraction of polysaccharide from peony seed cake was optimized. According to single factor experiment, the significances of nine factors(pH, celluase dosage, enzymolysis time, enzymolysis temperature, ultrasonic time, ultrasonic power, ultrasonic temperature, ratio of liquid to solid and particle size)affected the extraction quantity of polysaccharide were evaluated by PB design. Then the extraction conditions were optimized by BBD response surface methodology, and the antioxidant activity of peony seed polysaccharide was evaluated by DPPH method. The results showed that the optimal extraction conditions of polysaccharide from the peony seed cake were obtained as follows: pH 4.5, celluase dosage 0.45%, enzymolysis time 60 min, enzymolysis temperature 45?℃, ultrasonic time 19 min, ultrasonic power 300 W, ultrasonic temperature 40?℃, ratio of liquid to solid 19∶?1 and particle size 60 meshes. Under these conditions，the extraction quantity of polysaccharide was 196.87 mg/g.The peony seed polysaccharide had certain scavenging activity on DPPH free radical with IC50 31.19 μg/mL, but the scavenging activity was weaker than that of VC. |
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