| 王标诗，胡小军,张卫国，等.湿法超微粉碎结合水酶法提取辣木籽油及其氧化稳定性分析[J].中国油脂,2019,44(6):.[WANG Biaoshi, HU Xiaojun, ZHANG Weiguo，etc.Extraction of Moringa oleifera seed oil by aqueous enzymatic method combined with wet superfine comminution pretreatment and its oxidation stability[J].China Oils and Fats,2019,44(6):.] |
| 湿法超微粉碎结合水酶法提取辣木籽油及其氧化稳定性分析 |
| Extraction of Moringa oleifera seed oil by aqueous enzymatic method combined with wet superfine comminution pretreatment and its oxidation stability |
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| DOI： |
| 中文关键词: 湿法超微粉碎 水酶法 辣木籽油 提取 氧化稳定性 |
| 英文关键词:wet superfine comminution aqueous enzymatic method Moringa oleifera seed oil extraction oxidation stability |
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| 摘要点击次数: 1328 |
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| 中文摘要: |
| 以辣木籽为原料，经湿法超微粉碎预处理后再经过低温烘干制成辣木籽粉，通过水酶法提取辣木籽油。利用单因素实验研究料液比、pH、酶添加量、酶解时间、酶解温度对辣木籽油提取率的影响，在此基础上采用正交实验确定水酶法提取辣木籽油的最佳工艺条件；并以辣木籽油过氧化值为评价指标，考察光照、温度和抗氧化剂对辣木籽油氧化稳定性的影响。结果表明，辣木籽油最佳提取条件为：以中性蛋白酶和复合蛋白酶按1∶?1组成的复合酶为酶解用酶，料液比1∶?6，pH 5.0，复合酶添加量6%，酶解温度50?℃，酶解时间8 h。在最佳提取条件下，辣木籽油提取率为85.23%±0.72%。光照及高温均能使辣木籽油的过氧化值升高，其中光照比温度对辣木籽油过氧化值的影响更大。因此，贮藏辣木籽油时，应尽量放置低温、避光处。另外，添加抗氧化剂BHT也能有效提高辣木籽油的氧化稳定性。 |
| 英文摘要: |
| Moringa oleifera seeds were pretreated by wet superfine comminution, then dried at low temperature, and extracted by aqueous enzymatic method to make Moringa oleifera seed oil. On the basis of selecting neutral protease and compound protease(1∶?1) as complex enzyme, the influences of ratio of material to liquid, pH, dosage of enzyme, enzymolysis time and enzymolysis temperature on the extraction rate of Moringa oleifera seed oil were studied by single factor experiment, then the extraction conditions were optimized by orthogonal experiment. Moreover, the effects of light, temperature and antioxidant on the oxidation stability of Moringa oleifera seed oil were analyzed. The results showed that the optimal extraction conditions of Moringa oleifera seed oil were obtained as follows: ratio of material to liquid 1∶?6, pH 5.0, dosage of complex enzyme 6%, enzymolysis time 8 h, enzymolysis temperature 50?℃, and the extraction rate of Moringa ole ifera seed oil was 85.23%±0.72% under these conditions. Light and high temperature could cause the increase of peroxide value,in which the effect of light was greater than that of temperature during storage of Moringa oleifera seed oil. Therefore, Moringa oleifera seed oil should be placed at low temperature and no light during storage. In addition, BHT had good antioxidant effect on Moringa oleifera seed oil. |
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