| 董庆霖，王瑶，邢向英，等.淡紫拟青霉TD16促进雨生红球藻生长和油脂合成的研究[J].中国油脂,2019,44(8):.[DONG Qinglin，WANG Yao，XING Xiangying，etc.Paecilomyces lilacinus TD16 promoting cell growth and lipid synthesis of Haematococcus pluvialis[J].China Oils and Fats,2019,44(8):.] |
| 淡紫拟青霉TD16促进雨生红球藻生长和油脂合成的研究 |
| Paecilomyces lilacinus TD16 promoting cell growth and lipid synthesis of Haematococcus pluvialis |
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| DOI： |
| 中文关键词: 雨生红球藻 淡紫拟青霉TD16 混合培养 生物量 油脂 |
| 英文关键词:Haematococcus pluvialis Paecilomyces lilacinus TD16 mixed culture biomass lipid |
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| 中文摘要: |
| 为提高雨生红球藻生物量和油脂产率，将浓度分别为1×107个/mL和1.36×107个/mL的雨生红球藻与淡紫拟青霉TD16以不同比例混合培养，确定5∶?1为最佳藻菌接种体积比。雨生红球藻与淡紫拟青霉TD16混合培养与雨生红球藻单独培养相比：生物量（0.92 g/L）和平均生长速率（62.30 mg/(L·d)）分别提高24.32%和31.66%，倍增时间由4.26 d缩短至3.24 d；油脂产率(184.91 mg/L)和平均合成速率（13.21 mg/(L·d)）分别提高45.59%和45.64%，脂肪酸组成中C16∶?0和C18∶?1分别上升20.56%和19.27%；最高叶绿素a和虾青素质量浓度达到3.89 mg/L和4.37 mg/L,分别提高20.81%和35.71%。说明淡紫拟青霉TD16与雨生红球藻混合培养能够促进雨生红球藻的生长，提高油脂产率。 |
| 英文摘要: |
| In order to improve the biomass and lipid productivity of Haematococcus pluvialis, the H. pluvialis with the concentration of 1×107 cells/mL and Paecilomyces lilacinus TD16 with the concentration of 1.36×107 cells/mL were mixed and cultured in different proportions, and the optimal inoculation volume ratio was 5∶?1. Compared with individual culture, H. pluvialis in mixed culture：the biomass (0.92 g/L) and average growth rate (62.30 mg/(L·d)) increased by 24.32% and 31.66% respectively, while doubling time shortened from 4.26 d to 3.24 d; lipid productivity (184.91 mg/L) and the average lipid synthesis rate (13.21 mg/(L·d)) increased by 45.59% and 45.64% respectively, C16∶?0 and C18∶?1 contents increased by 20.56% and 19.27%, respectively; the highest mass concentrations of chlorophyll a (3.89 mg/L) and astaxanthin (4.37 mg/L) increased by 20.81% and 35.71% respectively. These results demonstrated that the mixed culture of P. lilacinus TD16 and H. pluvialis could promote the cell growth and lipid synthesis. |
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