| 常振刚1，刘彦君2，潘丽2，李茜2，李亚菲2，马鸣远2，张林3.植物甾醇油酸酯-虾青素复合脂质体的制备工艺优化[J].中国油脂,2023,48(6):.[CHANG Zhengang1, LIU Yanjun2, PAN Li2, LI Xi2,
LI Yafei2, MA Mingyuan2, ZHANG Lin3.Optimization of preparation process of liposomes co-encapsulating phytosterol oleate and astaxanthin[J].China Oils and Fats,2023,48(6):.] |
| 植物甾醇油酸酯-虾青素复合脂质体的制备工艺优化 |
| Optimization of preparation process of liposomes co-encapsulating phytosterol oleate and astaxanthin |
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| DOI： |
| 中文关键词: 虾青素 植物甾醇油酸酯 复合脂质体 包封率 响应面法 |
| 英文关键词:astaxanthin phytosterol oleate composite liposomes encapsulation efficiency response surface methodology |
| 基金项目:国家自然科学基金青年科学基金项目（32001738）；郑州市科技局自然科学项目（21ZZXTCX18）；河南工业大学青年骨干教师培育计划项目；省部级科研平台开放课题（GO202209）；许昌市英才计划项目 |
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| 中文摘要: |
| 为了改善虾青素的水溶性和稳定性，探索植物甾醇酯用于构建负载虾青素脂质体的可行性，以大豆磷脂为主要膜材，采用薄膜-超声法制备植物甾醇油酸酯-虾青素复合脂质体（L-PA）。以包封率为评价指标，通过单因素试验和Box-Behnken响应面试验优化L-PA的制备工艺。结果表明：L-PA制备的最佳工艺条件为植物甾醇油酸酯与大豆磷脂质量比1∶ 10、磷酸盐缓冲液pH 7.4、超声时间4 min、吐温80与大豆磷脂质量比1∶ 2、有机相与水相体积比1∶ 4、虾青素与大豆磷脂质量比1∶ 50，在此条件下L-PA的包封率为95.24%。因此，植物甾醇油酸酯可用于构建负载虾青素的脂质体。 |
| 英文摘要: |
| In order to enhance the stability and water solubility of astaxanthin, and to explore the feasibility of phytosterol esters for preparing astaxanthin-loaded liposomes, liposomes co-encapsulating phytosterol oleate and astaxanthin (L-PA) were prepared by thin-film ultrasonic method with soybean phospholipid as the main membrane material. The preparation process of L-PA was optimized by single factor experiment and Box-Behnken response surface methodology with the encapsulation efficiency as the index. The results showed that the optimal process conditions were obtained as follows: mass ratio of phytosterol oleate to soybean phospholipid 1∶ 10, pH of phosphate buffer 7.4, ultrasonic time 4 min, mass ratio of Tween 80 to soybean phospholipid 1∶ 2, volume ratio of organic phase to aqueous phase 1∶ 4, and mass ratio of astaxanthin to soybean phospholipid 1∶ 50. Under these conditions, the encapsulation efficiency of L-PA was 95.24%. Therefore, phytosterol oleate can be used to prepare astaxanthin-loaded composite liposomes. |
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