| 胡韶华，张星河，卢永茂，陶冠军，韦伟，金青哲，王兴国.乳中脂溶性维生素A、D、E、K的快速检测方法研究[J].中国油脂,2025,50(6):.[HU Shaohua, ZHANG Xinghe, LU Yongmao, TAO Guanjun, WEI Wei,
JIN Qingzhe, WANG Xingguo.Fast analysis of fat-soluble vitamins A, D, E and K in milk[J].China Oils and Fats,2025,50(6):.] |
| 乳中脂溶性维生素A、D、E、K的快速检测方法研究 |
| Fast analysis of fat-soluble vitamins A, D, E and K in milk |
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| DOI：10.19902/j.cnki.zgyz.1003-7969.240173 |
| 中文关键词: 超高效液相色谱 母乳 脂溶性维生素 哺乳动物乳 婴幼儿配方奶粉 |
| 英文关键词:ultra-high performance liquid chromatography human milk fat-soluble vitamins mammalian milk infant formula |
| 基金项目: |
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| Author Name | Affiliation | | HU Shaohua, ZHANG Xinghe, LU Yongmao, TAO Guanjun, WEI Wei,
JIN Qingzhe, WANG Xingguo | （School of Food Science and Technology, Jiangnan University, Wuxi 214122, Jiangsu, China) |
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| 中文摘要: |
| 旨在为哺乳期母亲营养需求的确定和婴幼儿配方奶粉的设计提供参考数据，建立了一种超高效液相色谱（UPLC）搭配光电二极管阵列检测器（PDA）分析母乳中脂溶性维生素A、D、E、K含量的方法，对其进行了方法学验证，并采用该方法检测了母乳、其他哺乳动物乳（牛乳、羊乳、驴乳）及配方奶粉中维生素A、D、E、K的含量，同时对不同样品中脂溶性维生素含量进行了主成分分析（PCA）。结果表明：所建立的UPLC-PDA方法可在10 min内实现对不同脂溶性维生素的分离；各脂溶性维生素的标准曲线回归方程均具有良好的线性关系（R2≥0.999 4），检出限和定量限范围分别为0.01~0.40 μg/mL和0.09~1.38 μg/mL，精密度和准确度的计算结果均不大于5%，除维生素K受到碱法前处理影响导致加标回收率偏低外，其他脂溶性维生素的加标回收率范围（80.69%~111.66%）均处于良好水平；母乳中维生素A含量介于羊乳与牛乳间，维生素E的含量高于其他哺乳动物乳，但明显低于配方奶粉，且母乳及其他哺乳动物乳中均未检出维生素D；PCA结果显示，其他哺乳动物乳（尤其是羊乳）和母乳中脂溶性维生素组成及含量相近。综上，UPLC-PDA方法适用于乳和配方奶粉中脂溶性维生素含量的测定。 |
| 英文摘要: |
| To provide reference data for determining the nutritional needs of lactating mothers and designing infant formula, a method for analyzing the contents of fat-soluble vitamins A, D, E, and K in human milk using ultra-high performance liquid chromatography (UPLC) coupled with a photodiode array detector (PDA) was established, and the method was validated. The UPLC-PDA method was applied to detect the contents of vitamins A, D, E, and K in human milk, other mammalian milks(cow milk, goat milk, and donkey milk) and infant formula. A principal component analysis (PCA) of the fat-soluble vitamin contents in different samples were also performed. The results indicated that the established UPLC-PDA method achieved separation of different fat-soluble vitamins within 10 min. The regression equations of the standard curves for each fat-soluble vitamin showed good linearity (R2≥0.999 4), with detection limits and quantification limits ranging from 0.01 μg/mL to 0.40 μg/mL and 0.09 μg/mL to 1.38 μg/mL, respectively. The calculation resluts for precision and accuracy were not greater than 5%. Except for vitamin K, which was affected by the alkaline pretreatment resulting in a lower spiked recovery rate, the spiked recovery rates for the other fat-soluble vitamins ranged from 80.69% to 111.66%, indicating good levels. Vitamin A content in human milk was between that of goat milk and cow milk, and the vitamin E content was higher than that in other mammalian milks, but significantly lower than that in infant formula. Vitamin D was not detected in human milk or other mammalian milks. PCA results showed that the composition and content of fat-soluble vitamins in mammalian milks, especially goat milk, was similar to that in human milk. In conclusion, UPLC-PDA method is suitable for analyzing the content of fat-soluble vitamins in milk and infant formula. |
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